RNA isolation from highly viscous samples rich in polyphenols and polysaccharides
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The homogenized solution of leaves from woody plantCinnamomum tenuipilum is relatively viscous, gluelike, and rich in polysaccharides and polyphenols, which makes the isolation of RNA particularly difficult. If drawing with a tip, continuous filaments are formed at the end of tip. Guanidine-based and phenol/SDS methods resulted in brown and water-insoluble RNA pellets. With modifications of a CTAB method by including 2 centrifugation steps at 30,000g, we developed an effective and reproducible protocol that yields enough good quality RNA for northern analysis and RT-PCR.