Plant Molecular Biology Reporter

, Volume 20, Issue 4, pp 399–406 | Cite as

Purification of plastid DNA from an enriched rhodoplast fraction of the red algaGracilaria tenuistipitata

  • Jonathan C. Hagopian
  • Pi Nyvall
  • Mariana C. de Oliveira


We report a straightforward protocol for isolating plastid DNA from an enriched rhodoplast fraction of the red algaGracilaria tenuistipitata. Plastids were purified using differential centrifugation and 2-step sucrose density gradients. We found that 10% polyethylene glycol 4000 was essential for maintaining plastid integrity prior to lysis. Plastid DNA isolated directly from the purified rhodoplasts was sufficiently pure for restriction endonuclease fragment analyses. Database comparisons of sequences generated randomly from a shotgun genomic library indicated that plastid DNA was 89% pure following ultracentrifugation in isopycnic cesium chloride equilibrium gradients. The protocol yields 30–50 μg of plastid DNA per 100 g of fresh algal tissue.

Key words

Gracilaria tenuistipitata plastid isolation plastid DNA purification ptDNA red alga Rhodophyta 



nuclear DNA


plastid DNA


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Copyright information

© Springer 2002

Authors and Affiliations

  • Jonathan C. Hagopian
    • 1
  • Pi Nyvall
    • 2
  • Mariana C. de Oliveira
    • 1
  1. 1.Departamento de Botânica, Instituto de BiociênciasUniversidade de São PauloSão PauloBrazil
  2. 2.UMR 1931, CNRS-Goëmar, Station BiologiqueCNRS-INSU-Université Paris 6Roscoff CedexFrance

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