Journal of Assisted Reproduction and Genetics

, Volume 14, Issue 2, pp 102–110

Strategies for ovulation induction and oocyte retrieval in the lowland gorilla

  • Harry H. Hatasaka
  • Nan E. Schaffer
  • Phillip E. Chenette
  • Wlodzimierz Kowalski
  • Bryan R. Hecht
  • Thomas P. Meehan
  • Anne Colston Wentz
  • Rafael F. Valle
  • Robert T. Chatterton
  • Rajasingam S. Jeyendran
Animal Experimentation

DOI: 10.1007/BF02765779

Cite this article as:
Hatasaka, H.H., Schaffer, N.E., Chenette, P.E. et al. J Assist Reprod Genet (1997) 14: 102. doi:10.1007/BF02765779

Abstract

Purpose: Ovulation induction and oocyte retrieval were performed in a lowland gorilla in an attempt to propagate and potentially cryopreserve embryos from an infertile animal and to advance techniques to help preserve this endangered species.

Results: Following 34 days of leuprolide acetate suppression, human menopausal gonadotropins were administered for 14-days in a 32-year-old wild-born lowland gorilla. Ten oocytes were retrieved by transrectal ultrasound-guided aspiration. Other approaches to oocyte recovery were not feasible in this case. A serum estradiol concentration of 4700 pg/ml at the time of human chorionic gonadotropin administration did not induce ovarian hyperstimulation. Mature oocytes were recovered from follicles measuring 14 to 24 mm in diameter, with a corresponding average serum estradiol concentration of approximately 300 pg/ml for each mature follicle. Cryopreservation of a gorilla embryo was effected from cryopreserved gorilla spermatozoa.

Conclusions: Parameters for monitoring ovulation induction in the gorilla appear to be similar to those for humans. The results indicate that the use of a gonadotropin releasing hormone agonist and higher doses of gonadotropins than previously used in gorillas appear to improve oocyte recovery.

Key words

lowland gorilla infertility assisted reproductive techniques in vitro fertilization 

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Harry H. Hatasaka
    • 1
  • Nan E. Schaffer
    • 3
    • 4
  • Phillip E. Chenette
    • 1
  • Wlodzimierz Kowalski
    • 1
  • Bryan R. Hecht
    • 1
  • Thomas P. Meehan
    • 3
  • Anne Colston Wentz
    • 1
  • Rafael F. Valle
    • 1
  • Robert T. Chatterton
    • 1
  • Rajasingam S. Jeyendran
    • 1
    • 4
  1. 1.Section of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Prentice Women’s HospitalNorthwestern University Medical SchoolChicago
  2. 2.Department of Obstetrics and GynecologyUniversity of Utah School of MedicineSalt Lake City
  3. 3.Lincoln Park ZooChicago
  4. 4.Andrology Laboratory ServicesChicago
  5. 5.San Francisco Center for Reproductive MedicineSan Francisco
  6. 6.College of MedicineNortheastern Ohio UniversitiesCanton
  7. 7.Andrology Laboratory ServicesChicago

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