Molecular Biotechnology

, Volume 5, Issue 1, pp 39–47 | Cite as

Cloning single-stranded DNA

  • Joachim Messing


DNA sequence and expression analyses have greatly benefited from using M13 and pUC derived cloning vectors and their polycloning sites. A chronology of the original concepts and experiments is reviewed.

Index Entries

M13mp and pUC history polycloning sites universal sequencing primer 


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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Joachim Messing
    • 1
  1. 1.Waksman Institute, RutgersThe State University of NJPiscataway

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