Deletion of theAlu-VpA/MycL1 (1p34.3) locus is a negative prognostic sign in human colorectal cancer
We examined deletions of the short arm of chromosome 1 and aberrations of the microsatellite locusAlu-VpA/MycL1 (1p34.3) in human primary colorectal adenocarcinomas. Cytogenetically discernible deletions in 1p were found in 45% (14/31) of informative tumors. The 1p-tumors commonly exhibited a polyploid karyotype (FisherP 1=0.023) and a larger number of rearranged chromosomes (P 2=0.045) versus those without 1p deletions. The 1p deletions often combined with chromosome 5 monosomy (χ2=6.24; p=0.013), chromosome 15 monosomy (χ2=4.20;p=0.040), and 11q deletions (P 2=0.035). Among the 50 carcinomas, 11 (22%) showedAlu-VpA/MycL1 instability, and 14% (6/43 informative) had lost theAlu-VpA/MycL1 allele. The genetic alterations thus revealed were collated with the clinical and morphological features of the tumors. The loss of the 1p material was shown to be correlated with marked karyotype aberrations in colorectal tumors, andAlu-VpA/MycL1 allele deletions were tightly associated with relapses or metastasis within 30 months after surgery.
Key wordscolorectal cancer karyotype 1p locus microsatellite repeat loss of heterozygosity Alu-VpA/MycL1 PCR prognostic sign
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