Molecular Biology

, Volume 34, Issue 5, pp 652–654 | Cite as

Millimolar concentrations of methylmethanesulfonate enhance transcription from the cytomegalovirus early promoter at least 100-fold

  • E. N. Nabirochkina
  • A. V. Ivanov
  • A. V. Soldatov
Ten Years of the Institute of Gene Biology
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Abstract

Treatment of cultured cells with methylmethanesulfonate enhanced transcription from the CMV early promoter 25–250 times and protein synthesis 60–100 times in a dose-dependent way. The effect was promoter-specific and was not observed with a retroviral LTR promoter. Structural analogs such as ethylmethanesulfonate, trifluoromethanesulfonate, andp-toluenesulfonate did not enhances expression from the CMV promoter.

Key words

transcription regulation CMV promoter/enhancer cell culture methylmethanesulfonate 
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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • E. N. Nabirochkina
    • 1
    • 3
  • A. V. Ivanov
    • 2
    • 3
  • A. V. Soldatov
    • 1
    • 4
  1. 1.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  3. 3.Center of Medical ResearchUniversity of OsloMoscowRussia
  4. 4.Max-Planck-Institut fur Molekulare GenetikBerlinGermany

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