Potato Research

, Volume 48, Issue 3–4, pp 191–200 | Cite as

Potato cultivar identification using simple sequence repeats markers (SSR)

  • M. Moisan-Thiery
  • S. Marhadour
  • M. C. Kerlan
  • N. Dessenne
  • M. Perramant
  • T. Gokelaere
  • Y. Le Hingrat
Full Papers


Identification of potato cultivars is currently based on phenotypic characters. Crop inspections are needed at different stages and the increasing number of cultivars means the process is becoming more and more complex. Molecular markers are a possible complementary tool to identify potato cultivars and to rapidly check the identity of seeds lots. In this study 286 potato cultivars produced in France were characterized by Polymerase Chain Reaction (PCR) using Simple Sequence Repeats (SSR) markers. Sequential amplifications with 4 to 5 of the chosen SSR markers enabled complete discrimination between all the cultivars. The patterns were registered in a database and the procedure is now used routinely in France.

Additional keywords

Genetic fingerprint microsatellites polymorphism Solarium tuberosum subsp.tuberosum 


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  1. Akkaya, M.S., A.A. Bhagwat & P.B Cregan, 1992. Length polymorphism of Simple Sequence Repeat DNA in soybean.Genetics 132: 1131–1139.PubMedGoogle Scholar
  2. Aranzana, M.J., J. Carbó & P. Arús, 2003. Microsatellite variability in peach (Prunus persica L. Batsch): cultivar identification, marker mutation, pedigree inferences and population structure.Theoretical and Applied Genetics 106: 1341–1352.PubMedGoogle Scholar
  3. Bassam, B.J., G. Caetano-Anolles & P.M. Gresshoff, 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels.Annals of Biochemistry 196: 80–83.CrossRefGoogle Scholar
  4. Becher, S.A., K. Steinmer, K. Weising, S. Boury, D., Peltier, J.-P. Renou, G. Kahl & K. Wolff, 2000. Microsatellites for cultivar identification inPelargonium.Theoretical and Ap-plied Genetics 101: 643–651.CrossRefGoogle Scholar
  5. Demecke, T., L.M. Kawchuk & D.R. Lynch, 1993. Identification of potato cultivars and clonal variants by random amplified polymorphic DNA analysis.American Potato Journal 70: 561–570.Google Scholar
  6. Douches, D.S. & K. Ludlam, 1991. Electrophoretic characterization of North American potato cultivars.American Potato Journal 68: 767–780.Google Scholar
  7. Esselink, G.D., M.J.M. Smulders & B. Vosman, 2002. Identification of cut rose (Rosa kybrida) and rootstock varieties using robust sequence tagged microsatellite site markers.Theoretical and Applied Genetics 106(2): 277–286.PubMedGoogle Scholar
  8. Esselink, G.D., H. Nybom & B. Vosman, 2004. Assignment of allelic configuration in polyploids using the MAC-PR (microsatellite DNA allele counting-peak ratios) method.Theoretical and Applied Genetics 109(2): 402–408.PubMedCrossRefGoogle Scholar
  9. Gebhardt, C., C. Blomendhal, U. Schachtschabel, T. Debener, F. Salamini & E. Ritter, 1989. Identification of 2n breeding lines and 4n varieties of potato (Solanum tuberosum ssp.tuberosum) with RFLP fingerprinting.Theoretical and Applied Genetics 78: 16–22.CrossRefGoogle Scholar
  10. Ghislain, M., D.M. Spooner, F. Rodriguez, F. Villamon, J. Nunez, C. Vasquez, R. Waugh & M. Bonierbale, 2004. Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato.Theoretical and Applied Genetics 108: 881–890.PubMedCrossRefGoogle Scholar
  11. Görg, R., U. Schachtschabel, E. Ritter, F. Salamini & C. Gebhardt, 1992. Discrimination among 136 Tetraploid potato varieties by fingerprints using highly polymorphic DNA markers.Crop Science 32: 15–819.CrossRefGoogle Scholar
  12. Hosaka, K., M. Mori & K. Ogawa, 1994. Genetic relationships of Japanese potato cultivars assessed by RAPD analysis.American Journal of Potato Research 71: 535–546.CrossRefGoogle Scholar
  13. Jones, C.J., K.J. Edwards, S. Castaglione, M.O. Winfield, F. Sala, C. van de Wiel, G. Bredemeijer, B. Vosman, M. Matthes, A. Daly, R. Brettschneider, P. Bettini, M. Buiatti, E. Maestri, A. Malcevschi, N. Marmiroli, R. Aert, G. Volckaert, J. Rueda, R. Linacero, A. Vazquez & A. Karp, 1997. Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories.Molecular Breeding 3: 381–390.CrossRefGoogle Scholar
  14. Kawchuk, L.M., D.R. Lynch, J. Thomas, B. Penner, D. Sillito & F. Kulcsar, 1996. Characterization ofSolanum tuberosum simple sequence repeats and application to potato cultivar identification.American Potato Journal 73: 325–335.Google Scholar
  15. McGregor, C.E., C.A. Lambert, M.M. Greyling, J.H. Louw & L. Warnich, 2000. A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm.Euphytica 113: 135–144.CrossRefGoogle Scholar
  16. Milbourne, D., R. Meyer, J.E. Bradshaw, E. Baird, N. Bonar, J. Provan, W. Powell & R. Waugh, 1997. Comparison of PCR-based marker system for the analysis of genetic relationships in cultivated potato.Molecular Breeding 3: 127–136.CrossRefGoogle Scholar
  17. Milbourne, D., R.C. Meyer, A.J. Collins, L.D. Ramsay, C. Gebhardt & R. Waugh, 1998. Isolation, characterisation and mapping of simple sequence repeat loci in potato.Molecular Genetics and Genomics 259: 233–245.Google Scholar
  18. Moisan-Thiery, M., Y. Le Hingrat & M.C. Kerlan. 2000. Potato cultivars identification using molecular markers. In: C. Dore, F. Dosba & C. Baril (Eds), Procedings of the International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture.Acta Horticulturae, vol. 546, ISHS, pp. 471–477.Google Scholar
  19. Mori, M., K. Hosaka, Y. Umemura & C. Kaneda, 1993. Rapid identification of Japanese potato cultivars by RAPDs.Japanese Journal of Genetics 68: 167–174.CrossRefGoogle Scholar
  20. Norero, N., J. Malleville, M. Huarte & M. Feingold, 2002. Cost efficient potato (Solanum tuberosum L.) cultivar identification by microsatellite amplification.Potato Research 45: 131–138.CrossRefGoogle Scholar
  21. Nybom, H., G.D. Esselink, G. Werlemark & B. Vosman, 2004. Microsatellite DNA marker inheritance indicates preferential pairing between two highly homologous genomes in polyploid and hemisexual dog-roses,Rosa L. SectCaninae DC.Heredity 92: 139–150.PubMedCrossRefGoogle Scholar
  22. Oliver, J.L. & J.M. Martinez-Zapater, 1985. A genetic classification of potato cultivars based on allozyme patterns.Theoretical and Applied Genetics 69: 305–311.CrossRefGoogle Scholar
  23. Prevost, A. & M.J. Wilkinson, 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars.Theoretical and Applied Genetics 98: 107–112.CrossRefGoogle Scholar
  24. Provan, J., W. Powell & R. Waugh, 1996. Microsatellite analysis of relationships within cultivated potato (Solanum tuberosum).Theoretical and Applied Genetics 92: 1078–1084.CrossRefGoogle Scholar
  25. Sosinski, B. & D.S. Douches, 1996. Using polymerase chain reaction-based DNA amplification to fingerprint north american potato cultivars.HorstScience 31(1): 130–133.Google Scholar
  26. Zhang, D., C. Besse, M.Q. Cao & M.H. Gandelin, 2001. Evaluation of AFLPs for variety identification in modern rose (Rosa hybrida L.). Eds. Doré, Dosba et Baril.Acta Horticulturae 546: 351–357.Google Scholar

Copyright information

© Kluwer Academic Publishers 2005

Authors and Affiliations

  • M. Moisan-Thiery
    • 1
  • S. Marhadour
    • 1
  • M. C. Kerlan
    • 2
  • N. Dessenne
    • 3
  • M. Perramant
    • 4
  • T. Gokelaere
    • 5
  • Y. Le Hingrat
    • 6
  1. 1.Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche APBV Agrocampus RennesFédération Nationale des Producteurs de Plant de Pommes de Terre (FNPPPT)PloudanielFrance
  2. 2.Unité Mixte de Recherche APBV Agrocampus RennesINRAPloudanielFrance
  3. 3.Comité Nord Plants de Pommes de terre (CNPPT)BeaurainsFrance
  4. 4.RoudouhirBretagne PlantsHanvecFrance
  5. 5.FNPPPTParisFrance
  6. 6.FNPPPT RoudouhirHanvecFrance

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