Molecular Biotechnology

, Volume 10, Issue 3, pp 273–274 | Cite as


A vector for direct cloning of PCR products in a double Xcm1 restriction site offering compatible single 3′-overhanging T residues
  • Erik de Vries
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The mutiple cloning site of pUC19 was replaced by a multiple cloning site possessing a double Xcm1 restriction site. Digestion withXcmI gives a linear vector with a single 3′-overhanging T-residue at both ends. This provides the easiest way of creating a vector in which PCR fragments produced byTaq polymerase can be directly cloned without further modifications.


ApaI Multiple Cloning Site White Coloni Linear Vector Phage Cloning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  1. 1.Department of Parasitology and Tropical Veterinary MedicineUtrecht UniversityUtrechtThe Netherlands

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