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The mutiple cloning site of pUC19 was replaced by a multiple cloning site possessing a double Xcm1 restriction site. Digestion withXcmI gives a linear vector with a single 3′-overhanging T-residue at both ends. This provides the easiest way of creating a vector in which PCR fragments produced byTaq polymerase can be directly cloned without further modifications.
KeywordsApaI Multiple Cloning Site White Coloni Linear Vector Phage Cloning
- 4.Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989)Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Plainview, New York.Google Scholar