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Molecular Biotechnology

, Volume 10, Issue 3, pp 273–274 | Cite as

pUCPCR1

A vector for direct cloning of PCR products in a double Xcm1 restriction site offering compatible single 3′-overhanging T residues
  • Erik de Vries
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Abstract

The mutiple cloning site of pUC19 was replaced by a multiple cloning site possessing a double Xcm1 restriction site. Digestion withXcmI gives a linear vector with a single 3′-overhanging T-residue at both ends. This provides the easiest way of creating a vector in which PCR fragments produced byTaq polymerase can be directly cloned without further modifications.

Keywords

ApaI Multiple Cloning Site White Coloni Linear Vector Phage Cloning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Marchuk, D., Drumrn, M., Saulino, A., and Collins, F. S. (1991) Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.Nucleic Acids Res. 19, 1154.PubMedCrossRefGoogle Scholar
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    Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.Gene 33, 103–119.PubMedCrossRefGoogle Scholar
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    Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989)Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Plainview, New York.Google Scholar

Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  1. 1.Department of Parasitology and Tropical Veterinary MedicineUtrecht UniversityUtrechtThe Netherlands

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