Molecular Neurobiology

, Volume 11, Issue 1–3, pp 223–230 | Cite as

The effect of methamphetamine on the mRNA level for 14·3·3 ⥈ chain in the human cultured cells

  • Tatsuyuki Muratake
  • Shigenobu Hayashi
  • Yuka Ichimura
  • Ken Morii
  • Ryozo Kuwano
  • Tomio Ichikawa
  • Toshiro Kumanishi
  • Toshiaki Isobe
  • Masahiko Watanabe
  • Hisatake Kondo
  • Yasuo Takahashi
Proceedings of the Symposium Cellular and Molecular Mechanisms of Drugs of Abuse Cocaine and Methamphetamine held in Nice, France, August 19–20, 1993

Abstract

14·3·3 protein, a brain-specific protein, is an activator of tyrosine and tryptophan hydroxylases, key enzymes for biosynthesis of dopamine and serotonin. In this article, we describe cloning of cDNA for human brain 14·3·3 ν chain and expression of 14·3·3 ν chain mRNA in some human cultured cells. The cloned cDNA is 1730 bp long and contains 191 bp of a 5′-noncoding region, the complete 738 bp of coding region, and 801 bp of a 3′-noncoding region, containing three polyadenylation signals. This cDNA encoded a polypeptide of 246 amino acids (M, 28,196). Furthermore, usingin situ hybridization histochemistry, the expression of mRNA for this protein was examined in the rat central nervous system.In situ hybridization histochemistry indicated that 14·3·3 ⥈ chain mRNA is detected not only in the monoamine-synthetic neurons, but also in other neurons in the discrete nuclei, which synthesize neither cathecholamine nor serotonin. Northern blot analysis demonstrated that the addition of methamphetamine into the cultured medium increased the mRNA level for 14·3·3 ⥈ chain in U-251 cells, but did not increase that of GFAP.

Index Entries

14·3·3 protein human cell lines cDNA mRNA methamphetamine in situ hybridization 

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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Tatsuyuki Muratake
    • 1
  • Shigenobu Hayashi
    • 1
  • Yuka Ichimura
    • 2
  • Ken Morii
    • 2
  • Ryozo Kuwano
    • 2
  • Tomio Ichikawa
    • 3
  • Toshiro Kumanishi
    • 3
  • Toshiaki Isobe
    • 4
  • Masahiko Watanabe
    • 5
  • Hisatake Kondo
    • 5
  • Yasuo Takahashi
    • 1
    • 3
  1. 1.National Saigata HospitalOhgata Town, Nakakubikigun, Niigata PrefectureJapan
  2. 2.Research Laboratory for Molecular GeneticsNiigata UniversityNiigata CityJapan
  3. 3.Brain Research InstituteNiigata UniversityNiigata CityJapan
  4. 4.Faculty of ScienceTokyo Metropolitan UniversityHachioji City, TokyoJapan
  5. 5.Department of AnatomyTohoku University School of MedicineSendaiJapan

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