Molecular Neurobiology

, Volume 11, Issue 1–3, pp 67–76

Prenatal cocaine exposure revealed minimal postnatal changes in rat striatal dopamine D2 receptor sites and mRNA levels in the offspring

  • Alfreda Stadlin
  • Heung Ling Choi
  • Karl Wah Keung Tsim
  • David Tsang
Proceedings of the Symposium Cellular and Molecular Mechanisms of Drugs of Abuse Cocaine and Methamphetamine held in Nice, France, August 19–20, 1993

Abstract

It has been reported from this laboratory that prenatal cocaine exposure results in the postnatal transient alterations of rat striatal dopamine uptake sites examined from postnatal 0–32 wk. The present study aims to examine whether this will result in a direct/indirect stimulation of dopamine D2 receptors. Pregnant rats were dosed orally with cocaine hydrochloride (60 mg/kg/d) from gestational day (GD) 7–21. Control animals received an equivalent volume of water. The striatum from the offspring at postnatal 0–32 wk was examined. The radioligand [3H]sulpiride was used for the Scatchard analysis of the D2 receptors, and the changes in the levels of mRNA for the D2 receptor were studied using Northern blot analysis. Results from the present study revealed that in the control group, there was an age-dependent increase in the number of D2 receptor sites (Bmax:44.00±2.12 to 178.00±45.10 fmol/mg protein) and in the levels of D2 mRNA from PN0–32 wk with the most rapid increase occurring during the first 4 wk of postnatal development. Prenatal cocaine exposure resulted in only a significant decrease (p<0.001) in the number of D2 receptor sites at PN0 wk and in a 10% increase in mRNA levels at PN3, 4, and 12 wk. It was concluded from this study that prenatal cocaine exposure resulted in minimal postnatal changes in the dopamine D2 receptor.

Index Entries

Cocaine prenatal dopamine D2 receptor [3H]sulpiride striatum rat 

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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Alfreda Stadlin
    • 1
  • Heung Ling Choi
    • 1
  • Karl Wah Keung Tsim
    • 3
  • David Tsang
    • 2
  1. 1.Department of AnatomyThe Chinese University of Hong KongShatin, New TerritoriesHong Kong
  2. 2.Department of BiochemistryThe Chinese University of Hong KongShatin, New TerritoriesHong Kong
  3. 3.Department of BiologyThe Hong Kong University of Science and TechnologyKowloonHong Kong

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