Endocrine

, Volume 5, Issue 2, pp 169–177 | Cite as

Effects of neuropeptide Y (NPY) and NPY agonists on lordosis in the female guinea pig

  • Janice E. Thornton
  • Laurie Holcomb
  • Sarah Leupen
  • Linda Kimbrough
Original Articles
Received:
Revised:
Accepted:

Abstract

We have previously shown that an NPY antagonist decreases lordosis behavior and that this decrease can be reversed with NPY administration. The present experiments examined whether intracerebroventricular (icv) administration of NPY would facilitate lordosis behavior and whether it would increase feeding behavior in the female guinea pig. Additionally, we examined whether icv administration of a more specific NPY Y1 and/or Y2 receptor agonist would facilitate lordosis behavior. Although NPY (1 μg) increased feeding behavior when it was administered to the lateral ventricle of ovariectomized (ovx) estrogen (i.e., estradiol benzoate; EB) and progesterone- (P) treated guinea pigs, it had no facilitatory effect on lordosis behavior at any of the doses tested (0.5, 1, 5, or 10μg). In fact, the lower doses had a small, delayed inhibitory effect. NPY also had no effect on lordosis in females treated with EB alone. In contrast, the NPY Y1 agonist (Leu31Pro34) NPY significantly facilitated lordosis in ovx EB- and P-treated females. It had no effect in ovx females treated with EB alone. The NPY Y2 agonist NPY (13–36) had a slight, delayed inhibitory effect in ovx EB- and P-treated females. These data are consistent with the hypothesis that NPY can act at a number of receptor subtypes to affect lordosis behavior, and that NPY can facilitate lordosis behavior by acting at Y1 receptors. Furthermore, it appears that this facilitatory effect of Y1 receptors is an effect on some progesterone-mediated component of lordosis, as the Y1 agonist facilitated EB- and P-induced lordosis, but not that induced with EB alone.

Key Words

Neuropeptide Y lordosis guinea pig reproduction feeding estrogen progesterone 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bauer, C. and Thornton, J. E. (1995).Soc. Neurosci. 21, 2093.Google Scholar
  2. Bauer-Dantoin, A. C., McDonald, J. K., and Levine, J. E. (1992).Endocrinology 131, 2946–2952.PubMedCrossRefGoogle Scholar
  3. Bauer-Dantoin, A. C., Urban, J. H., and Levine, J. E. (1992).Endocrinology 131, 2953–2958.PubMedCrossRefGoogle Scholar
  4. Bauer-Dantoin, A. C., Tabesh, B., Norgle, J. R., and Levine, J. E. (1993).Endocrinology 133, 2418–2423.PubMedCrossRefGoogle Scholar
  5. Besecke, L. M., Wolfe, A. M., Pierce, M. E., Takahashi, J. S., and Levine, J. E. (1994).Endocrinology 135, 1621–1627.PubMedCrossRefGoogle Scholar
  6. Bonavera, J. J., Dube, M. G., Kalra, P. S., and Kalra, S. P. (1994).Endocrinology 134, 2367–2370.PubMedCrossRefGoogle Scholar
  7. Brown, T. J. and Blaustein, J. D. (1984).Brain Res. 301, 343–349.PubMedCrossRefGoogle Scholar
  8. Bruning, J. L. and Kintz, B. L. (1968).Computational Handbook of Statistics. Scott Foresman: Glenview.Google Scholar
  9. Chronwell, B. M., DiMaggio, D. A., Massari, V. J., Pickel, V. M., Ruggiero, D. A., and Donohue, T. L. (1985).Neuroscience 15, 1159–1181.CrossRefGoogle Scholar
  10. Clark, J. T., Kalra, P. S., and Kalra, S. P. (1985).Endocrinology 117, 2435–2442.PubMedGoogle Scholar
  11. Collins, V. J., Boling, J. L., Dempsey, E. W., and Young, W. C. (1938).Endocrinology 23, 188–196.Google Scholar
  12. Crowley, W. R., Tessel, R. E., O'Donohue, T. L., Adler, B. A., and Kalra, S. P. (1985).Endocrinology 117, 1151–1155.PubMedGoogle Scholar
  13. Crowley, W. R., Hassid, A., and Kalra, S. P. (1987).Endocrinology 120, 941–945.PubMedGoogle Scholar
  14. Dempsey, E. W., Hertz, R., and Young, W. C. (1936).Am. J. Physiol. 116, 201–209.Google Scholar
  15. deQuidt, M. E. and Emson, P. C. (1986).Neuroscience 15, 1149–1157.Google Scholar
  16. Dumont, Y., Martel, J.-C., Fournier, A., St-Pierre, S., and Quirion, R. (1992).Progr. Neurobiol. 38, 125–167.CrossRefGoogle Scholar
  17. Fuhlendroff, L., Gether, U., Aakerlund, L., Langeland-Johansen, N., Thogersen, H., Melberg, S. G., Olsen, U. B., Thastrup, O., and Schwartz, W. T. (1990).Proc. Natl. Acad. Sci. USA 87, 182–186.CrossRefGoogle Scholar
  18. Goldfoot, D. A. and Goy, R. W. (1970).J. Comp. Physiol. Psychol. 62, 426–434.CrossRefGoogle Scholar
  19. Goy, R. W. and Young, W. C. (1957).Behaviour 10, 340–354.Google Scholar
  20. Grundemar, L., Wahlestedt, C., and Reis, D. J. (1991).J. Pharmacol. Exp. Ther. 258, 633–638.PubMedGoogle Scholar
  21. Grundemar, L., Sheikh, S. P., and Wahlestedt, C. (1993). In:The Biology of Neuropeptide Y and Related Peptides. Colmers, W. F. and Wahlestedt, C. (eds). Humana: Totowa, NJ, pp. 197–239.Google Scholar
  22. Herzog, H., Hart, Y. J., Shine, J., and Selbie, L. A. (1993).DNA Cell Biol. 12, 465–471.PubMedCrossRefGoogle Scholar
  23. Kalra, S. P. and Crowley, W. R. (1984).Life Sci. 35, 1173–1176.PubMedCrossRefGoogle Scholar
  24. Kalra, S. P. and Crowley, W. R. (1992).Frontiers Neuroendocrinology 13, 1–46.Google Scholar
  25. Kalra, S. P., Fuentes, M., Fournier, A., Parker, S. L., and Crowley, W. R. (1992).Endocrinology 130, 3323–3330.PubMedCrossRefGoogle Scholar
  26. Luparello, T. J. (1967).Stereotaxic Atlas of the Forebrain of the Guinea Pig. Williams and Wilkins: Baltimore, MD.Google Scholar
  27. McDonald, J. K., Lumpkin, M. D., Samson, W. K., and McCann, S. M. (1985).Proc. Natl. Acad. Sci. USA 82, 561–564.PubMedCrossRefGoogle Scholar
  28. Morali, G. and Beyer, C. (1979). In:Endocrine Control of Sexual Behavior. Beyer, C. (ed.). Raven Press: New York, pp. 33–75.Google Scholar
  29. Pfaff, D. and Schwartz-Giblin, S. (1988). In:The Physiology of Reproduction. Knobil, E. and Neill, J. (eds). Raven Press: New York, pp. 1487–1568.Google Scholar
  30. Rimland, J., Xin, W., Sweetnam, P., Saijoh, K., Nestler, E. J., and Duman, R. S. (1991).Mol. Pharmacol. 40, 869–875.PubMedGoogle Scholar
  31. Rubin, B. and Barfield, R. (1983).Endocrinology 113, 797–804.PubMedCrossRefGoogle Scholar
  32. Sabatino, F. D., Collins, P., and McDonald, J. K. (1990).Neuroendocrinology 52, 600–607.PubMedGoogle Scholar
  33. Sahu, A., Crowley, W. R., and Kalra, S. P. (1994).Endocrinology 134, 1018–1022.PubMedCrossRefGoogle Scholar
  34. Sheikh, S. P., Hakanson, R., and Schwartz, T. W. (1989).FEBS Lett. 245, 209–214.PubMedCrossRefGoogle Scholar
  35. Stanley, B. G. and Leibowitz, S. F. (1985).Proc. Natl. Acad. Sci USA 82, 3940–3943.PubMedCrossRefGoogle Scholar
  36. Stanley, B. G. (1993). In:The Biology of Neuropeptide Y and Related Peptides. Colmers, W. F. and Wahlestedt, C. (eds). Humana: Totowa, NJ, pp. 457–509.Google Scholar
  37. Tatemoto, K., Mann, M. J., and Shimuzu, M. (1992).Proc. Natl. Acad. Sci. USA 89, 1174–1178.PubMedCrossRefGoogle Scholar
  38. Thornton, J. E., Wallen, K., and Goy, R. W. (1987).Physiol. Behav. 40, 703–709.PubMedCrossRefGoogle Scholar
  39. Thornton, J. E., Goy, R. W., McEwen, B. S., and Feder, H. H. (1989).Pharm. Biochem. Behav. 32, 421–424.CrossRefGoogle Scholar
  40. Thornton, J. E. and Carson, S. (1995).Endocrine 3, 807–811.CrossRefGoogle Scholar
  41. Wahlestedt, C., Yanaiara, N., and Hakanson, R. (1986).Regul. Pept. 13, 307–318.PubMedCrossRefGoogle Scholar
  42. Wahlestedt, C., Edvinsson, L., Ekblad, E., and Hakanson, R. (1987). In:Neuronal Mesengers in Vascular Function. Fernstrom Symp. No. 10. Nobin, A. and Owman, C. H. (eds.). Elsevier: Amsterdam. pp. 231–242.Google Scholar
  43. Wahlestedt, C., Regunathan, S., and Reis, D. J. (1991).Life Sci. 50, 7–12.Google Scholar
  44. Wehrenberg, W. B., Corder, R., and Gaillard, R. C. (1989).Neuroendocrinology 49, 680–682.PubMedGoogle Scholar
  45. Young, W. C., Dempsey, E. C., and Meyers, H. I. (1935).J. Comp. Physiol. Psychol. 19, 313–335.Google Scholar
  46. Young, W. C., Dempsey, E. W., Hagquist, C. W., and Boling, J. L. (1937).J. Lab. Clin. Med. 23, 300–303.Google Scholar
  47. Young, W. C. (1961). In:Sex and Internal Secretions. Young, W. C. (ed.). Williams and Wilkins. Baltimore, pp. 1173–1239.Google Scholar

Copyright information

© Humana Press Inc 1996

Authors and Affiliations

  • Janice E. Thornton
    • 1
    • 2
  • Laurie Holcomb
    • 1
  • Sarah Leupen
    • 1
  • Linda Kimbrough
    • 1
  1. 1.Neuroscience/Biopsychology ProgramOberlin CollegeOberlin
  2. 2.Department of BiologyOberlin CollegeOberlin

Personalised recommendations