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Experimental Brain Research

, Volume 100, Issue 3, pp 385–394 | Cite as

Seizure suppression in kindling epilepsy by intracerebral implants of GABA-but not by noradrenalinereleasing polymer matrices

  • Merab Kokaia
  • Patrick Aebischer
  • Eskil Elmér
  • Johan Bengzon
  • Peter Kalén
  • Zaal Kokaia
  • Olle Lindvall
Original Paper

Abstract

Gamma-aminobutyric acid (GABA)-releasing polymer matrices were implanted bilaterally, immediately dorsal to the substantia nigra, in rats previously kindled in the amygdala. Two days after implantation, rats with GABA-releasing matrices exhibited only focal limbic seizures in response to electrical stimulation, whereas animals with control matrices devoid of GABA had generalized convulsions. GABA release from the polymer matrices was high during the first days after implantation, as demonstrated both in vitro and, using microdialysis, in vivo. The anticonvulsant effect was no longer observed at 7 and 14 days at which time GABA release was found to be low. In a parallel experiment, polymer matrices containing noradrenaline (NA) were implanted bilaterally into the hippocampus of rats with extensive forebrain NA depletion induced by an intraventricular 6-hydroxydopamine injection. No effect on the development of hippocampal kindling was observed, despite extracellular NA levels exceeding those of rats with intrahippocampal locus coeruleus grafts that have previously been shown to retard kindling rate. The results indicate that GABA-releasing implants located in the substantia nigra region can suppress seizure generalization in epilepsy, even in the absence of synapse formation and integration with the host brain. In contrast, the failure of NA-releasing polymer matrices to retard the development of seizures in NA-depleted rats suggests that such an effect can only be exerted by grafts acting through a well-regulated, synaptic release of NA.

Key words

Epilepsy GABA Noradrenaline Graft Rat 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Merab Kokaia
    • 1
  • Patrick Aebischer
    • 2
  • Eskil Elmér
    • 1
  • Johan Bengzon
    • 1
  • Peter Kalén
    • 3
  • Zaal Kokaia
    • 1
  • Olle Lindvall
    • 1
  1. 1.Restorative Neurology Unit, Department of NeurologyUniversity HospitalLundSweden
  2. 2.Division Autonome de Recherche ChirurgicaleCentre Hospitalier Universitaire VaudoisLausanneSwitzerland
  3. 3.Department of Medical Cell ResearchUniversity of LundLundSweden

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