The Indian Journal of Pediatrics

, Volume 64, Issue 4, pp 435–440

Molecular diagnosis of infectious diseases

Basic and Behavioural Sciences
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Abstract

The Polymerase Chain Reaction (PCR) is a revolutionary new means of amplifying,i.e., replicating, selected DNA sequencesin vitro. This procedure is highly sensitive and rapid, requiring few hours for detection of amplified target DNA whereas it often takes several weeks for the identification of many fastidious organisms by culture. Studies have shown that as few as one microorganism can be detected by PCR. Therefore, it is revolutionizing our ability in the diagnosis of infectious diseases. Currently, this technique is very expensive and complex which will probably restrict its immediate role for diagnosing infections where the causative organism is impossible to culture or untypeable by conventional means. As further progress occurs, however, this powerful technique will be applied as a major new tool to diagnose infections and to the study of individual genome variations.

Key Words

Infectious diseases Polymerase Chain Reaction (PCR) DNA probe DNA sequence Molecular biology 

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Copyright information

© Dr. K C Chaudhuri Foundation 1997

Authors and Affiliations

  1. 1.Texas Department of HealthRabies-Arbovirus Section, Medical Virology BranchUSA
  2. 2.AustinUSA

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