Journal of Molecular Neuroscience

, Volume 8, Issue 2, pp 159–161

Cloning and identification of human syntaxin 5 as a synaptobrevin/VAMP binding protein

  • Veerasamy Ravichandran
  • Paul A. Roche
Original Articles


Syntaxins are transmembrane proteins that function in regulating transport vesicle docking and fusion with target membranes in neuronal and nonneuronal cells. Vesicle docking is thought to be regulated in part by the specific interactions of syntaxin with a vesicle-associated membrane protein termed synaptobrevin/VAMP. We have cloned a 1557-bp cDNA that encodes the human syntaxin 5 isoform, using a combination of PCR and colony-screening methods. The deduced 301 amino-acid sequence of human syntaxin 5 shares 96% identity with rat syntaxin 5. Like rat syntaxin 1A, human syntaxin 5 binds to synaptobrevin/VAMP in vitro. The identification of human syntaxin 5 as a synaptobrevin/VAMP-binding protein supports the hypothesis that syntaxin 5 regulates protein transport by binding to vesicle-associated membrane proteins.

Index Entries

Syntaxin synaptobrevin SNAP-25 vesicular transport 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bennett M. K., Garcia-Arrarás J. E., Elferink L. A., Peterson K., Fleming A. M., Hazuka C. D., and Scheller R. H. (1993) The syntaxin family of vesicular transport receptors.Cell 74, 863–873.PubMedCrossRefGoogle Scholar
  2. Dascher C., Matteson J., and Balch W. E. (1994) Syntaxin 5 regulates endoplasmic reticulum to Golgi transport.J. Biol. Chem. 269, 29,363–29,366.Google Scholar
  3. Hata Y. and Südhof T. C. (1995) A novel ubiquitous form of Munc-18 interacts with multiple syntaxins.J. Biol. Chem. 270, 13,022–13,028.Google Scholar
  4. Ravichandran V., Chawla A., and Roche P. A. (1996) Identification of a novel syntaxin- and synaptobrevin/VAMP binding protein, SNAP-23, expressed in non-neuronal tissues.J. Biol. Chem. 271, 13,300–13,303.Google Scholar
  5. Rothman J. E. (1994) Mechanisms of intracellular protein transport.Nature 372, 55–63.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc 1997

Authors and Affiliations

  • Veerasamy Ravichandran
    • 1
  • Paul A. Roche
    • 1
  1. 1.Experimental Immunology Branch, National Cancer InstituteNational Institutes of HealthBethesda

Personalised recommendations