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Estuaries

, Volume 28, Issue 1, pp 160–172 | Cite as

Adapting the CHEMTAX method for assessing phytoplankton taxonomic composition in Southeastern U.S. estuaries

  • Alan J. LewitusEmail author
  • David L. White
  • Raphael G. Tymowski
  • Mark E. Geesey
  • Sabrina N. Hymel
  • Peter A. Noble
Article

Abstract

CHEMTAX is a matrix factorization program used to derive taxonomic structure of phytoplankton from photosynthetic pigment vitios. The program was originally developed from and applied to the analysis of oceanic phytoplankton assemblages. We found that application of the original CHEMTAX reference matrix to southeastern United States estuarine systems produced inaccurate results, as verified by microscopy. Modification of the matrix, based primarily on the pigment ratios of 33 estuarine isolates, improved the predictive capabilities of CHEMTAX for our samples. Limitations of the method included an overstimation of diatom biomass (due to the inability to differentiate diatoms from taxa with chloroplasts derived from diatom endosymbionts, notably some dinoflagellates) and a tendency to exclude some raphidophyte species. In complement with microscopic verification, the method was shown to improve assessment of phytoplankton taxonomic composition.

Keywords

Phytoplankton Pigment Composition North Inlet Accessory Pigment Pigment Ratio 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Estuarine Research Federation 2005

Authors and Affiliations

  • Alan J. Lewitus
    • 1
    Email author
  • David L. White
    • 2
  • Raphael G. Tymowski
    • 3
  • Mark E. Geesey
    • 4
  • Sabrina N. Hymel
    • 1
  • Peter A. Noble
    • 5
  1. 1.Bell W. Baruch Institute for Marine and Coastal SciencesUniversity of South Carolina, and Marine Resources Research Institute, South Carolina Department of Natural Resources, Hollings Marine LaboratorySouth CarolinaCharleston
  2. 2.Marine Science ProgramUniversity of South CarolinaColumbia
  3. 3.Baruch Marine LaboratoryBelle W. Baruch Institute for Marine and Coastal Sciences, University of South CarolinaGeorgetown
  4. 4.Grice Marine LaboratoryCollege of CharlestonCharleston
  5. 5.Civil and Environmental Engineering DepartmentUniversity of WashingtonSeattle98195

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