Dengue virus infection among children with undifferentiated fever in Karachi
- 135 Downloads
Sera were collected from a total of 122 children, comprising 117 cases with undifferentiated fever and 5 cases with dengue hemorrhagic fever (DHF), during June to September 1994 in Karachi, Pakistan. Sera were tested by the IgM-capture ELISA using dengue type 1 (D1), dengue type 2 (D2), West Nile (WN), and Japanese encephalitis (JE) viral antigens. Among 92 single sera from undifferentiated fever cases, IgM antibodies were detected in 5 cases by D1, 8 cases by D2, and 5 cases by WN antigens, respectively. Corresponding number of positives among 25 paired sera from undifferentiated fever cases were 3 by D1, 6 by 02, and 1 by WN antigen. Four out of 5 DHF cases possessed anti-D1 as well as anti-D2 IgM antibodies. Only a single DHF case was positive for anti-WN IgM antibodies. Anti-JE IgM antibodies were not detected in any of the tested serum specimens.
Clinical manifestations of undifferentiated fever patients were generally non specific, but the percentage of children with anemia, hepatomegaly and splenomegaly was higher in patients possessing anti-dengue IgM antibodies than those without. Among the groups with anti-dengue IgM antibodies, those possessing only anti-D2 but not anti-D1 IgM antibodies showed higher percentages with cough, edema, and splenomegaly.
The results indicated that up to 26% of the undifferentiated fever cases were caused by dengue virus infection in Karachi, Pakistan.
Key wordsOndifierentiated fever Dengue virus
Unable to display preview. Download preview PDF.
- 1.World Health Organization. Mosquitoborne hemorrhagic fevers of Southeast Asia and Western Pacific.Bull WHO 1966; 35:17–33.Google Scholar
- 3.Halstead, SB. The XXth century dengue pandemic: need for surveillance and research.Wld Hlth Statist Quart 1992; 45 : 292–298.Google Scholar
- 5.Wengler G. Family Flaviviridae. In : R.I. B. Francki, In R.I. B. Francki, C.M. Fauquet, D.L. Knudson, F. Brown (eds.).Classification and Nomenclature of Viruses : Springer-Verlag, Wien, New York.Arch Virol Suppl 2, pp 223–233.Google Scholar
- 9.Banerjee K. Dengue fever in rural areas of Maharashtra and Gujarat States in India.Dengue News 1990; 15: 3–5.Google Scholar
- 10.Mehardale SM, Risbud AR,et al. Outbreak of dengue fever in rural areas of Parbhani district of Maharashtra (India).Ind J Med Res (A), 1991; 93: 6–11.Google Scholar
- 11.Uddin MJ, Jalee S, Sharma RS, and Veghee T. Dengue fever/dengue haemorrhagic fever outbreak in Shahjahanpur, Uttar Pradesh, India, 1992.Dengue News Letter 1992; 18:2–3.Google Scholar
- 13.Wilson MB, and Nakane PK. Recent development in the peroxidase method of conjugating horseradish peroxidase (HRPO) to antibodies. In : W Knapp, K Holubar, and G, Wick (eds).Immunofluorescence and Related Stainwg Teehniques. Elsevier/North Holland ’ Biochemicals Press, Amsterdam pp. 215–224.Google Scholar