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Establishment and characterization of a bovine mammary epithelial cell line with unique properties

  • Boris Zavizion
  • Marilyn van Duffelen
  • Warren Schaeffer
  • Ioannis Politis
Cellular Models

Summary

Clonal cell lines (BME-UV) were established from primary epithelial cells by stable transfection with a plasmid, carrying the sequence of the simian virus 40 early region mutant tsA58, encoding the thermolabile large T antigen. The BME-UV cells have undergone more than 300 population doublings and produce intranuclear large T antigen. At low confluency, growing islands of cells are apparent exhibiting the characteristic cobblestone morphology of epithelial cells. The BME-UV cells expressed functional markers such as microvilli and desmosomes and biochemical markers of mammary epithelial cells such as a repertoire of cytokeratins. The BME-UV cells are capable of synthesizing low levels of α-lactalbumin and α8l (50 ng/ml of medium/24 h). One of the cell lines, BME-UV1 showed enhanced proliferation in the presence of epidermal growth factor (EGF) and insulinlike growth factor I (IGF-I). The BME-UV1 cell line is the only known bovine mammary epithelial cell line responsive to EGF. The BME-UV cells grown on collagen at low confluency are capable of developing very long projections that most likely allow for communication between cells at a distance from each other. The BME-UV cells may become a valid model system to examine bovine mammary epithelial proliferation and differentiation and cell-to-cell communication.

Key words

immortalization T-antigen 

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Copyright information

© Society for In Vitro Biology 1996

Authors and Affiliations

  • Boris Zavizion
    • 1
  • Marilyn van Duffelen
    • 1
  • Warren Schaeffer
    • 2
  • Ioannis Politis
    • 1
  1. 1.Department of Animal and Food SciencesUniversity of VermontBurlington
  2. 2.Department of Microbiology and Molecular GeneticsUniversity of VermontBurlington

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