Somatic Cell and Molecular Genetics

, Volume 23, Issue 3, pp 221–223 | Cite as

Assignment of the human gene encoding eukaryotic initiation factor 4E (EIF4E) to the region q21-25 on chromosome 4

  • Robin M. Jones
  • Marcy E. MacDonald
  • John Branda
  • Michael R. Altherr
  • David N. Louis
  • Emmett V. Schmidt
Brief Communication


We recently cloned genomic sequences containing the promoter region for the messenger RNA cap binding protein (eIF4E). As the rate-limiting step in translation, eukaryotic initiation factor 4E is important in cellular growth control. Using oligonucleotide primers specific for the promoter region in polymerase chain reactions (PCR), we amplified the human gene in a chromosome 4-specific human/rodent somatic cell panel. This panel mapped single copy genomic sequences for eIF4E in the region 4q21 to 4q25.


Polymerase Chain Reaction Acute Myeloid Leukemia Polymerase Chain Reaction Analysis Somatic Cell Hybrid Panel Specific Malignancy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Literature Cited

  1. 1.
    Merrick, W.C. (1992).Microbiological Reviews 56: 291–315.PubMedGoogle Scholar
  2. 2.
    Hershey, J.W. (1991).Ann. Rev. Biochem. 60:717–755.PubMedCrossRefGoogle Scholar
  3. 3.
    Hiremath, L.S., Webb, N.R., and Rhoads, R.E. (1985).J. Biol. Chem. 260:7843–7849.PubMedGoogle Scholar
  4. 4.
    Duncan, R., Milburn, S.C., and Hershey, J.W. (1987).J. Biol. Chem. 262:380–388.PubMedGoogle Scholar
  5. 5.
    Sonenberg, N. (1994).Biochimie 76:839–841.PubMedCrossRefGoogle Scholar
  6. 6.
    Smith, M.R., Jaramillo, M., Liu, Y.L., Dever, T.E., Merrick, W.C., Kung, H.F., and Sonenberg, N. (1990).New Biologist 2:648–654.PubMedGoogle Scholar
  7. 7.
    Koromilas, A.E., Lazaris-Karatzas, A., and Sonenberg, N. (1992).Embo J. 11:4153–4158.PubMedGoogle Scholar
  8. 8.
    Fagan, R.J., Lazaris-Karatzas, A., Sonenberg, N., and Rozen, R. (1991).J. Biol. Chem. 266:16518–16523.PubMedGoogle Scholar
  9. 9.
    Rosenwald, I.B., Lazaris-Karatzas, A., Sonenberg, N., and Schmidt, E.V. (1993).Mol. Cell. Biol. 13:7358–7363.PubMedGoogle Scholar
  10. 10.
    Lazaris-Karatzas, A., Montine, K.S., and Sonenberg, N. (1990).Nature 345:544–547.PubMedCrossRefGoogle Scholar
  11. 11.
    Lazaris-Karatzas, A., and Sonenberg, N. (1992).Mol. Cell. Biol. 12:1234–1238.PubMedGoogle Scholar
  12. 12.
    Kerekatte, V., Smiley, K., Hu, B., Smith, A., Gelder, F., and De Benedetti, A. (1995).International Journal of Cancer 64:27–31.CrossRefGoogle Scholar
  13. 13.
    Jones, R.M., Branda, J., Johnston, K.A., Polymenis, M., Gadd, M., Rustgi, A., Callanan, L., and Schmidt, E.V. (1996).Mol. Cell. Biol. 16:4754–4764.PubMedGoogle Scholar
  14. 14.
    Pelletier, J., Brook, J.D., and Housman, D.E. (1991).Genomics 10:1079–1082.PubMedCrossRefGoogle Scholar
  15. 15.
    Dorfman, J., Lazaris-Karatzas, A., Malo, D., Sonenberg, N., and Gros, P. (1991).Genomics 9:785–788.PubMedCrossRefGoogle Scholar
  16. 16.
    NIGMS Cambden, NJ.Google Scholar
  17. 17.
    Slaugenhaupt, S.A., Roca, A.L., Liebert, C.B., Altherr, M.R., Gusella, J.F., and Reppert, S.M. (1995).Genomics 27:355–357.PubMedCrossRefGoogle Scholar
  18. 18.
    Eubanks, J.H., Altherr, M., Wagner-McPherson, C., McPherson, J.D., Wasmuth, J.J., and Evans, G.A. (1992)Genomics 12:510–516.PubMedCrossRefGoogle Scholar
  19. 19.
    Waha, A., Rollbrocker, B., Wiestler, O.D., and von Deimling, A. (1996).Diagnostic Molecular Pathology 5:147–150.PubMedCrossRefGoogle Scholar
  20. 20.
    Bigner, S.H., Mark, J., Mahaley, M.S., and Bigner, D.D. (1984).Hereditas 101:103–113.PubMedGoogle Scholar
  21. 21.
    Mitelman, F. (1994).Catalog of Chromosome Aberrations in Cancer. Wiley-Liss, New York,pp. 4252.Google Scholar
  22. 22.
    Heim, S., and Mitelman, F. (1995).Cancer Cytogenetics. Wiley-Liss, New York,pp. 536.Google Scholar
  23. 23.
    Das, S., Kearney, L., Bower, M., Chaplin, T., Riley, J.H., Anand, R., and Young, B.D. (1992).Genes, Chromosomes and Cancer 5:244–251.CrossRefGoogle Scholar
  24. 24.
    Cimino, G., Nakamura, T., Gu, Y., Canaani, O., Prasad, R., Crist, W.M., Carroll, A.J., Baer, M., Bloomfield, C.D., Nowell, P.C., and et al. (1992).Cancer Res. 52:3811–3813.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Robin M. Jones
    • 1
    • 2
  • Marcy E. MacDonald
    • 3
  • John Branda
    • 1
  • Michael R. Altherr
    • 4
  • David N. Louis
    • 5
  • Emmett V. Schmidt
    • 1
    • 2
  1. 1.Massachusetts General Hospital Cancer CenterCharlestown
  2. 2.The Children’s ServiceMassachusetts General HospitalBoston
  3. 3.Molecular Neurogenetics UnitMassachusetts General HospitalCharlestown
  4. 4.Genomics and Structural Biology GroupLos Alamos National LaboratoryLos Alamos
  5. 5.Department of PathologyMassachusetts General HospitalBoston

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