Influence of formamide on the thermal stability of DNA
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Abstract
The utility of formamide in the denaturation and renaturation of DNA has been examined. The melting temperature of duplex DNA is lowered by 0·6°C per per cent formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30%, it does not affect the rate of DNA reassociation
Keywords
DNA melting formamide Tm DNA base composition DNA reassociationAbbreviations used
- EDTA
Ethylenediamine-tetraacetic acid
- RNA
ribonucleic acid
- SDS
sodium dodecyl sulphate
- Ti
temperature of incubation
- Tm
temperature midpoint of melting
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© Indian Academy of Sciences 1984