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Journal of Biosciences

, Volume 14, Issue 3, pp 221–231 | Cite as

Comparison of biotin binding protein of pregnant rat serum with rat serum albumin

  • P. B. Seshagiri
  • P. R. Adiga
Article
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Abstract

The purified biotin binding protein of pregnant rat serum was shown to be immunologically similar to rat serum albumin as assessed by a sensitive radioimmunoassay. In radioimmunoassay for rat biotin binding protein, the binding of [125I] rat biotin binding protein to anti-chicken egg yolk biotin binding protein antibodies was displaced by both rat serum (10–100 nl) and purified rat serum albumin (0.1–10 ng). Similarly, in radioimmunoassay for rat serum albumin the binding of [125I] rat serum albumin to either anti-rat serum albumin antibodies or anti-chicken egg yolk biotin binding protein antibodies was displaced by unlabelled rat biotin binding protein at comparable concentration range (0·5–10 ng). Significant fractions of radioiodinated rat biotin binding protein and rat serum albumin bound to antibodies to chicken egg yolk biotin binding protein. In immature rats, the circulating half-lives of rat biotin binding protein and rat serum albumin were determined to be 12 and 17 h respectively. The rat biotin binding protein and rat serum albumin were analysed by techniques that exploit their physicochemical properties. They displayed similar electrophoretic mobilities in alkaline as well as denaturing sodium dodecyl sulphate-polyacrylamide gels. However, in nonequilibrium pH gradient polyacrylamide gel electrophoresis, they resolved clearly. In two-dimensional tryptic peptide map analysis, the two proteins showed similarities as well as significant differences in the relative distribution patterns of their iodopeptides. These results showed that the primary structure of rat biotin binding protein and rat serum albumin were different in finer details despite the fact that they shared significant immunological cross-reactivity.

Keywords

Biotin binding protein (chicken, rat) rat serum albumin, immunological-crossreactivity 

Abbreviations used

BBP

Biotin binding protein

rBBP

rat biotin-binding protein

RIA

radioimmunoassay

RSA

rat serum albumin

SDS

sodium dodecyl sulphate

PAGE

polyacrylamide gel electrophoresis

SA

serum albumin

Mr

molecular weight

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Copyright information

© Indian Academy of Sciences 1989

Authors and Affiliations

  • P. B. Seshagiri
    • 1
  • P. R. Adiga
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia

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