Lung

, Volume 162, Issue 1, pp 317–323 | Cite as

The immunocytochemical detection of angiotensin-converting enzyme in alveolar macrophages from patients with sarcoidosis

  • Yasuharu Takada
  • Kunio Hiwada
  • Hiroshi Akutsu
  • Akihide Hashimoto
  • Tatsuo Kokubu
Original Articles
Accepted:

Abstract

Angiotensin-converting enzyme (ACE) was detected in alveolar macrophages obtained by bronchoalveolar lavage (BAL), but not in lymphocytes and other cells, by immunofluorescence using anti-human ACE antibody. The enzyme was localized on the surface of plasma membrane and cytoplasmic processes of alveolar macrophages, as demonstrated by immunoelectron microscopy. The immunocytochemical staining for ACE in alveolar macrophages from patients with sarcoidosis was intense, which was consistent with the result that ACE activity in BAL cells from patients with sarcoidosis was significantly higher than in cells from normal subjects and from patients with nonsarcoid lung diseases.

Key words

Angiotensin-converting enzyme Sarcoidosis Alveolar macrophage Bronchoalveolar lavage Immunocytochemistry 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Yasuharu Takada
    • 1
  • Kunio Hiwada
    • 1
  • Hiroshi Akutsu
    • 2
  • Akihide Hashimoto
    • 1
  • Tatsuo Kokubu
    • 1
  1. 1.Second Department of Internal MedicineEhime University School of MedicineEhimeJapan
  2. 2.National Sanatrium Ehime HospitalEhimeJapan

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