Advertisement

Lung

, Volume 161, Issue 1, pp 47–55 | Cite as

Role of alveolar macrophages in development of paraquat-induced lung injury

  • T. Etoh
  • S. Shioya
  • Y. Ohta
  • H. Yamabayashi
  • J. Hata
Original Articles

Abstract

It is not known if alveolar macrophages (AM) play an important role in the development of interstitial changes in the lung. The purpose of the present study was to learn if AM are related to the initiation of paraquat-induced lung injury in rats. An intraperitoneal injection of 10 mg/kg paraquat was followed by a rapid increase in the number of viable AM. This effect reached a maximum at 24 h after injection. When 90 mg/kg of methylprednisolone was injected subcutaneously at the same time of the administration of paraquat there was no increase in the number of viable AM. After weekly injection of 5 mg/kg paraquat for six weeks, there were histological changes which were significantly supressed by the simultaneous administration of methylprednisolone. Methylprednisolone, however, did not show such suppressive effects when administered 4 d after the injection of paraquat. It is suggested that the emergence of viable AM is one of the key phenomena in the initiation of interstitial changes in paraquat-induced lung injury.

Key words

Lung Macrophage Paraquat Steroids 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ando M, Suga M, Sugimoto M, Tokuomi H (1979) Superoxide production in pulmonary alveolar macrophages and killing of BCG by superoxide-generating system with or without catalase. Infect Immun 24:404–410PubMedGoogle Scholar
  2. 2.
    Blussé van Oud Alblas A, Van der Linden-Schrever B, Mattie H, Van Furth R (1981) The effect of glucocorticosteroids on the kinetics of pulmonary macrophages. J Reticuloendothel Soc 30:1–14PubMedGoogle Scholar
  3. 3.
    Hamilton J, Vassalli JD, Reich E (1976) Macrophage plasminogen activator: induction by asbestos is blocked by anti-inflammatory steroids. J Exp Med 144: 1689–1694PubMedCrossRefGoogle Scholar
  4. 4.
    Hesterberg TW, Last JA (1981) Ozone-induced acute pulmonary fibrosis in rats. Prevention of increased rates of collagen synthesis by methylprednisolone. Am Rev Respir Dis 123:47–52PubMedGoogle Scholar
  5. 5.
    Hocking WG, Golde DW (1979) The pulmonary-alveolar macrophage. Part II. N Engl J Med 301:639–645PubMedCrossRefGoogle Scholar
  6. 6.
    Johnson KJ, Fantone JC III, Kaplan J, Ward PA (1981) In vivo damage of rat lungs by oxygen metabolites. J Clin Invest 67:983–993PubMedGoogle Scholar
  7. 7.
    Johnston RB Jr, Godzik CA, Cohn ZA (1978) Increased superoxide anion production by immunologically activated and chemically elicited macrophages. J Exp Med 148:115–127PubMedCrossRefGoogle Scholar
  8. 8.
    Jones PA, Werb Z (1980) Degradation of connective tissue matrices by macrophages. II. Influence of matrix composition on proteolysis of glycoproteins, elastin, and collagen by macrophages in culture. J Exp Med 152:1527–1536PubMedCrossRefGoogle Scholar
  9. 9.
    Leibovich SJ, Ross R (1976) A macrophage-dependant factor that stimulates the proliferation of fibroblasts in vitro. Am J Pathol 84:501–514PubMedGoogle Scholar
  10. 10.
    Martin WJ II, Gadek JE, Hunninghake GW, Crystal RG (1981) Oxidant injury of lung parenchymal cells. J Clin Invest 68::1277–1288PubMedCrossRefGoogle Scholar
  11. 11.
    Nathan CF, Murray HW, Cohn ZA (1980) The macrophage as an effector cell. N Engl J Med 303:622–626PubMedCrossRefGoogle Scholar
  12. 12.
    Smith P, Heath D, Kay JM (1974) The pathogenesis and structure of paraquat-induced pulmonary fibrosis in rats. J Pathol 114:57–67PubMedCrossRefGoogle Scholar
  13. 13.
    Stevens JB, Autor AP (1977) Oxygen-induced synthesis of superoxide dismutase and catalase in pulmonary macrophages of neonatal rats. Lab Invest 37:470–478PubMedGoogle Scholar
  14. 14.
    Thompson J, Van Furth R (1970) The effect of glucocorticosteroids on the kinetics of mononuclear phagocytes. J Exp Med 131:429–442PubMedCrossRefGoogle Scholar
  15. 15.
    Van Furth R, Cohn ZA (1968) The origin and kinetics of mononuclear phagocytes. J Exp Med 128:415–433PubMedCrossRefGoogle Scholar
  16. 16.
    Vijeyaratnam GS, Corrin B (1971) Experimental paraquat poisoning: a histological and electron-optical study of the changes in the lung. J Pathol 103:123–129PubMedCrossRefGoogle Scholar
  17. 17.
    Volkman A (1966) The origin and turnover of mononuclear cells in peritoneal exudates in rats. J Exp Med 124:241–253PubMedCrossRefGoogle Scholar
  18. 18.
    Weiss SJ, Lobuglio AF, Kessler HB (1980) Oxidative mechanisms of monocyte-mediated cytotoxicity. Proc Natl Acad Sci USA 77:584–587PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH & Co KG 1983

Authors and Affiliations

  • T. Etoh
    • 1
  • S. Shioya
    • 1
  • Y. Ohta
    • 1
  • H. Yamabayashi
    • 1
  • J. Hata
    • 2
  1. 1.Department of Internal MedicineTokai University School of MedicineBohseidai, IseharaJapan
  2. 2.Department of PathologyTokai University School of MedicineBohseidai, IseharaJapan

Personalised recommendations