Journal of Chemical Sciences

, 113:55 | Cite as

Small-angle neutron scattering study of sodium cholate and sodium deoxycholate interacting micelles in aqueous medium

  • J Santhanalakshmi
  • G Shantha Lakshmi
  • V K Aswal
  • P S Goyal
Article
Received:
Revised:

Abstract

Small angle neutron scattering (SANS) measurements of D2O solutions (0.1 M) of sodium cholate (NaC) and sodium deoxycholate (NaDC) were carried out atT= 298 K. Under compositions very much above the critical micelle concentration (CMC), the bile salt micelle size growths were monitored by adopting Hayter-Penfold type analysis of the scattering data. NaC and NaDC solutions show presence of correlation peaks atQ = 0.12 and 0.1 å-1 respectively. Monodisperse ellipsoids of the micelles produce best fits. For NaC and NaDC systems, aggregation number (9.0, 16.0), fraction of the free counterions per micelle (0.79, 0.62), semi-minor (8.0 å) and semi-major axes (18.4, 31.7 å) values for the micelles were deduced. Extent of micellar growth was studied using ESR correlation time measurements on a suitable probe incorporating NaC and NaDC micelles. The growth parameter (axial ratio) values were found to be 2.3 and 4.0 for NaC and NaDC systems respectively. The values agree with those of SANS.

Keywords

Biosurfactants sodium deoxycholate micelles bile salt micelles SANS study ESR correlation times 
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References

  1. 1.
    Esumi K and Ueno M (eds) 1997Structure-performance relationships in surfactants (Surfactant Science Series) (New York: Marcel Dekker) vol 70, chap. 3, pp 147–195Google Scholar
  2. 2.
    Small D M 1971The bile acid (New York: Plenum) vol 1, p. 302Google Scholar
  3. 3.
    Borgtstrom B, Barrowman J A and Lindstrom M 1985 InSterols and bile acid (eds) H Danielsson and J Sjoval (Amsterdam: Elsevier)Google Scholar
  4. 4.
    Small D M, Penkett S and Chapman D 1969Biochim. Biophys. Acta 176 178Google Scholar
  5. 5.
    Bourges M, Small D M and Dervichian D G 1967Biochim. Biophys. Acta 144 189Google Scholar
  6. 6.
    Small D M 1968J. Am. Oil Chem. Soc. 45 198Google Scholar
  7. 7.
    Oakenful D G and Fisher L R 1977J. Phys. Chem. 81 1838CrossRefGoogle Scholar
  8. 8.
    Zakrzewska J, Markovic V, Vucelic D, Feigin L, Dembo A and Mogilevsky L 1990J. Phys. Chem. 94 5079CrossRefGoogle Scholar
  9. 9.
    Esposito G, Giglio E, Pavel N V and Zanobi A 1987J. Phys. Chem. 91 356CrossRefGoogle Scholar
  10. 10.
    Hao L, Lu R, Least D G and Poulin P R 1997J. Solution Chem. 26 113Google Scholar
  11. 11.
    Coello A, Meijide F, Rodriguez Nunez E and Vazquez Tato J 1993J. Phys. Chem. 97 10186CrossRefGoogle Scholar
  12. 12.
    Pedersen J S, Egelhaaf S and Schurtenberger P 1995J. Phys. Chem. 99 1299CrossRefGoogle Scholar
  13. 13.
    Cohen D E, Thurston G M, Chamberlin R A, Benedek G B and Carey M C 1998Biochemistry 37 14798CrossRefGoogle Scholar
  14. 14.
    Conte G, Di Blasi R, Giglio E, Parretta A and Pavel N V 1984J. Phys. Chem. 88 5720CrossRefGoogle Scholar
  15. 15.
    O’Connor C J, Ch’ng B T and Wallace R G 1983J. Coll. Int. Sci. 95 410CrossRefGoogle Scholar
  16. 16.
    Coello A, Meijide F, Rodriguez Nunez E and Vazquez Tato J 1996J. Pharm. Sci. 85 9CrossRefGoogle Scholar
  17. 17.
    Hjelm R P, Schteingart C D, Hofmann A F and Thiagarajan P 2000J. Phys. Chem. B104 197Google Scholar
  18. 18.
    Lopez F, Samseth J, Mortensen K, Rosenqvist E and Rouch J 1996Langmuir 12 6188CrossRefGoogle Scholar
  19. 19.
    Igimi H and Carey M C 1980J. Lipid Res. 21 72Google Scholar
  20. 20.
    Goyal P S, Chakravarthy R, Dasannacharya B A, Desa J A E, Kelkar V K, Manohar C, Narasimhan S L, Rao K R and Valaulikar B S 1989Physica 156 471CrossRefGoogle Scholar
  21. 21.
    Goyal P S, Dasannacharya B A, Kelkar V K, Manohar C, Srinivasa Rao K and Valaulikar B S 1991Physica 174 196CrossRefGoogle Scholar
  22. 22.
    Kawamura H, Murata Y, Yamaguchi T, Igimi H, Tanaka M, Sugihara G and Kratohvil J P 1989J. Phys. Chem. 93 3321CrossRefGoogle Scholar
  23. 23.
    Kratohvil J P 1984Hepatology 4 85SCrossRefGoogle Scholar
  24. 24.
    Cannon B, Polnaszek C F, Butler K W, Erikson K E G and Smith I C P 1975Arch. Biochim. Biophys. 167 505CrossRefGoogle Scholar
  25. 25.
    Goyal P S, Aswal V K and Joshi V 1995 InUser’s manual for position sensitive detector based small angle neutron scattering spectrometer, Bhabha Atomic Research Centre, Mumbai, Government of IndiaGoogle Scholar
  26. 26.
    Berr S S 1987J. Phys. Chem. 91 4760CrossRefGoogle Scholar
  27. 27.
    Santhanalakshmi J, Goyal P S, Aswal V K and Vijayalakshmi G 1999Proc. Indian Acad. Sci. (Chem. Sci.) 111 651CrossRefGoogle Scholar
  28. 28.
    Young C Y, Missel P J, Mazer N A, Benedek G B and Carey M C 1978J. Phys. Chem. 82 1375CrossRefGoogle Scholar
  29. 29.
    Schreier S, Polnaszek C F and Smith I C P 1978Biochim. Biophys. Acta 515 375Google Scholar
  30. 30.
    Polnaszek C F 1976 InSpin labelling theory and applications (ed.) L J Berliner (New York: Academic Press)Google Scholar
  31. 31.
    Kooser R G, Volland W V and Freed J H 1969J. Chem. Phys. 50 5243CrossRefGoogle Scholar
  32. 32.
    Blow D M and Rich A 1959Nature (London) 182 3566Google Scholar
  33. 33.
    Esposito G and Giglio E 1987J. Phys. Chem. 91 356CrossRefGoogle Scholar
  34. 34.
    Small D M 1968Adv. Chem. Ser. 84 31CrossRefGoogle Scholar
  35. 35.
    Dembo A T, Sosfenov N I and Feigin L A 1966Sov. Phys. Crystallogr. (Engl. Transl.) 581 19Google Scholar
  36. 36.
    Mazer N A, Carey M C, Kwasnick R F and Benedek G B 1979Biochemistry 18 3064CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 2001

Authors and Affiliations

  • J Santhanalakshmi
    • 1
  • G Shantha Lakshmi
    • 1
  • V K Aswal
    • 2
  • P S Goyal
    • 2
  1. 1.Department of Physical ChemistryUniversity of MadrasChennaiIndia
  2. 2.Condensed Matter Physics DivisionBhabha Atomic Research DivisionMumbaiIndia

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