Korean Journal of Chemical Engineering

, Volume 20, Issue 6, pp 1073–1076 | Cite as

Caffeine Molecular Imprinted Microgel Spheres by Precipitation Polymerization

  • Dexian Wang
  • Seung Pyo Hong
  • Gengliang Yang
  • Kyung Ho Row


Imprinted uniform microgel spheres were prepared by precipitation polymerization. Acetonitrile was used as the dilute solvent with MAA as the monomer, EDMA as the crosslinker and caffeine as the print molecule. Comparison of caffeine adsorption on molecular imprinted and blank microgel spheres was made. Langmuir model was used to fit the adsorption data. It was found that the caffeine imprinted microgel spheres show specific binding sites to the target molecules. A binding study of caffeine on imprinted microgel spheres was made by Scatchard analysis; the dissociation constants (KD) and the maximum binding capacity were KD= 1.84×10−4mol/L,Q max = 16.98 μmol/g for high affinity binding site and KD=1.33×l0−3 mol/L, Qmax=46.84 μmol/g for lower affinity binding site, respectively This microgel spheres can be useful affinity adsorbents in further applications.

Key words

Molecular Imprinted Polymers Microgel Spheres Caffeine 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ansell, R. J., Kriz, D. and Mosbach, K.,“Molecularly Imprinted Polymers for Bioanalysis: Chromatography, Binding Assays and Biomimetic Sensors,”Curr. Opin. Biotechnol.,7, 89 (1996).CrossRefGoogle Scholar
  2. Bereczki, A., Tolokan, A., Horvai, G., Horvath, V, Lanza, F., Hall, A. J. and Sellergren, B.,“Determination of Phenytoin in Plasma by Molecularly Imprinted Solid-phase Extraction,”J. Chromatogr. A,930, 31 (2001).CrossRefGoogle Scholar
  3. Chen, W, Liu, F., Zhang, X, Li, K. A. and Tong, S,“The Specificity of a Chlorphenamine-imprinted Polymer and its Application,”Talanta,55, 29 (2001).CrossRefGoogle Scholar
  4. Chen, Y, Kele, M., Quinones, I., Sellergren, B. and Guichon, G.,“Influence of the pH on the Behavior of an Imprinted Polymeric Stationary Phase-Supporting Evidence for a Binding Site Mode,”J. Chromatogr. A,927, 1 (2001).CrossRefGoogle Scholar
  5. Haginaka, J. and Kagawa, C,“Uniformly Sized Molecularly Imprinted Polymer for d-Chlorpheniramine, Evaluation of Retention and Molecular Recognition Properties in an Aqueous Mobile Phase,”J. Chromatogr.A.,948, 77 (2002).CrossRefGoogle Scholar
  6. Hosoya, K., Yoshizako, K., Shirasu, Y, Kimata, K., Araki, T., Tanaka, N. and Haginaka, I,“Molecularly Imprinted Uniform-size Polymer-based Stationary Phase for High-performance Liquid Chromatography, Structural Contribution of Cross-linked Polymer Network on Specific Molecular Recognition,/rdJ. Chromatogr. A,728, 139 (1996).CrossRefGoogle Scholar
  7. Kriz, O., Ramstrom, O. andMosbach, K.,“Molecular Imprinting: New Possibilities for Sensor Technology,”Anal. Chem.,69, 345A (1997).Google Scholar
  8. Lang, C. D., Peng, H., Bao, X. Y, Nie, L. H. and Yao, S. Z.,“Study of a Molecular Imprinting Polymer Coated BAW Bio-mimic Sensor and its Application to the Determination of Caffeine in Human Serum and Urine,”Analyst,124, 1781 (1999).CrossRefGoogle Scholar
  9. Matsui, J., Miyoshi, Y., Doblhoff-Dier, O. and Takeuchi, T,“A Molecularly Imprinted Synthetic Polymer Receptor Selective for Atrazine,”Anal. Chem.,67, 4404 (1995).CrossRefGoogle Scholar
  10. Matsui, I, Nicholls, I. A. and Takeuchi, T,“Molecular Recognition in Cinchona Alkaloid Molecular Imprinted Polymer Rods”Analytica. Chimica. Acta.,365, 89 (1998).CrossRefGoogle Scholar
  11. Mayees, A. G. and Mosbach, K.,“Molecularly Imprinted Polymer Beads: Suspension Polymerization Using a Liquid Perfluorocarbon as the Dispersing Phase,”Anal. Chem.,68, 3769 (1996).CrossRefGoogle Scholar
  12. Owens, P. K., Karlsson, L., Lutz, E. S. M. and Andersson, L. I.,“Molecular Imprinting for Bio and Pharmaceutical Analysis,”Trends Anal. Chem.,18, 146 (1999).CrossRefGoogle Scholar
  13. Sajonz, P., Kele, M., Zhong, G., Sellergren, B. and Guiochon, G.,“Study of the Thermodynamics and Mass Transfer Kinetics of Two Enantiomers on a Polymeric Imprinted Stationary Phase,”J. Chromatogr. A,810, 1 (1998).CrossRefGoogle Scholar
  14. Sellergren, B. and Shea, K. J.,“Origin of Peak Asymmetry and the Effect of Temperature on Solute Retention in Enantiomer Separations on Imprinted Chiral Stationary Phases,”J. Chromatogr. A,690, 29 (1995).CrossRefGoogle Scholar
  15. Shernngton, D. C, “Recent Developments in Solid-phase Organic Synthesis,” Chem. Commun, 2275 (1998).Google Scholar
  16. Spivak, D. and Shea, K. J.,“Molecular Imprinting of Carboxylic Acids Employing Novel Functional Macroporous Polymers,”J. Org. Chem.,64, 4627 (1999).CrossRefGoogle Scholar
  17. Ye, L., Cormack, P. A. G. and Mosbach, K,“Molecuar Imprinting on Microgel Spheres,”Analytica Chimica Acta,435, 187 (2001).CrossRefGoogle Scholar
  18. Ye, L. and Mosbach, K.,“Molecularly Imprinted Microspheres as Antibody Binding Mimics,”Reactive & Functional Plymers,48, 149 (2001).CrossRefGoogle Scholar
  19. Yoshikawa, M., Fujisawa, T, Izumi, J., Kitao, T. and Sakamoto, S.,“Molecularly Imprinted Polymeric Membranes Involving Tetrapeptide EQKL Derivatives as Chiral-recognition Sites Toward Amino Acids,/rdAnal. Chim. Acta,365, 59 (1998).CrossRefGoogle Scholar
  20. Zhang, T, Liu, E, Chen, W., Wang, J. and Li, K.,“Influence of Intromolecular Hydrogen Bond of Templates on Molecular Recognition of Molecularly Imprinted Polymers,”Analytica Chimica Acta,450, 53 (2001).CrossRefGoogle Scholar
  21. Zheng, N, L1, Y. Z., Chang, W. B., Wang, Z. M. and L1, T. X, “Sulfonamide Imprinted Polymers using Co-functional Monomers,”Analytica Chimica Acta,452, 277 (2002).CrossRefGoogle Scholar
  22. Zhou, J., He, X. and Li Y,“Binding Study on 5,5-Diphenylhydantoin Imprinted Polymer Constructed by Utilizing an Amide Functional Group,”Analytica Chimica Acta,394, 353 (1999).CrossRefGoogle Scholar

Copyright information

© Korean Institute of Chemical Engineering 2003

Authors and Affiliations

  • Dexian Wang
  • Seung Pyo Hong
    • 1
  • Gengliang Yang
    • 2
  • Kyung Ho Row
    • 1
  1. 1.Center for Advanced Bioseparation Technology, Department of Chemical EngineeringInha UniversityIncheonKorea
  2. 2.Department of ChemistryHebei UniversityBaodingP. R. China

Personalised recommendations