Korean Journal of Chemical Engineering

, Volume 14, Issue 2, pp 109–116 | Cite as

Supercritical fluid extraction and bioassay identification of prodrug substances from natural resources

  • Min Jeong Noh
  • Eun Sun Choi
  • Seon Hee Kim
  • Ki-Pung Yoo
  • Young Hae Choi
  • Young Won Chin
  • Jinwoong Kim
Article
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Accepted:

Abstract

For arbitrarily chosen thirty types of natural resources which have been widely used in oriental traditional herb medicine, supercritical CO2 extraction (SFE) and organic liquid solvent extraction (LSE) withn-hexane, chloroform and methanol were carried out to extract pharmaceutical substances. To evaluate relative advantages and shortcomings between the SFE and LSE, five types of bioactivity assays as well as gas- and thin layer-chromatographic analysis were performed for all the extracts obtained by the two extraction methods. Types of bioassays performed included cytotoxicity, bleb forming, DNA binding, oxygen free radical scavenger and Xanthine oxidase inhibitor tests. To evaluate economic viability of the SFE over the traditional LSE, extractability of prodrug substances was evaluated as the functions of extraction temperature and pressure. SFE was proven to be a feasible alternative over LSE. Also, the optimum SFE conditions which provided maximum extraction and cytotoxicity for each selected sample were presented.

Key words

Supercritical Extraction Carbon Dioxide Pharmaceutical Substances Natural Plant Materials Bioassays Cytotoxicities 
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Copyright information

© Korean Institute of Chemical Engineering 1997

Authors and Affiliations

  • Min Jeong Noh
    • 1
  • Eun Sun Choi
    • 1
  • Seon Hee Kim
    • 1
  • Ki-Pung Yoo
    • 1
  • Young Hae Choi
    • 1
    • 2
  • Young Won Chin
    • 1
    • 2
  • Jinwoong Kim
    • 1
    • 2
  1. 1.Dept. of Chemical EngineeringSogang UniversitySeoulKorea
  2. 2.College of PharmacySeoul National UniversitySeoulKorea

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