Advertisement

Journal of Biosciences

, Volume 27, Issue 3, pp 261–272 | Cite as

Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

  • T. Venugopal
  • S. Mathavan
  • T. J. PandianEmail author
Article

Abstract

A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96–98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

Keywords

cDNA cloning growth hormone Indian major carps overexpression protein 3-D structure RACE 

Abbreviations used

GH

Growth hormone

GTC

guanidium thiocyanate-phenol-chloroform

H-RACE

homology based-rapid amplification of cDNA ends

PRL

prolactin

RACE

rapid amplification of cDNA ends

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abdel-Meguid S S, Shiegh H S, Smith W W, Dayringer H E, Violand B N and Bentle L A 1987 Three-dimensional structure of a genetically engineered variant of porcine growth hormone;Proc. Natl. Acad. Sci. USA 84 6434–6437PubMedCrossRefGoogle Scholar
  2. Agelon L B 1998 Promotion of rapid growth of rainbow trout (Salmo gairdneri) by a recombinant fish growth hormone;Can. J. Fish. Aquat. Sci. 45 146–151CrossRefGoogle Scholar
  3. Aramburo C, Navanette S, Monifiel J L, Rocio S and Berghman L R 1991 Purification and electrophoretic analysis of glycosylates chicken growth hormone (G-CGH): Evidence of G-CH isoforms;Gen. Comp. Endocrinol. 84 135–146PubMedCrossRefGoogle Scholar
  4. Ausubel F M, Brent R, Kingston R E, Moore P P, Seidman J G and Smith J A 1999Current protocols in molecular biology (New York: John Wiley)Google Scholar
  5. Bernardi G, D’Onofrio G, Caccio’ S and Bernardi G 1993 Molecular phylogeny of bony fishes based on their amino acid sequence of the growth hormone;J. Mol. Evol. 16 644–649Google Scholar
  6. Carlacci L and Chou K C 1990 Energetic approach to the folding of four α helix connected sequentially;Protein Eng. 3 509–514PubMedCrossRefGoogle Scholar
  7. Carlacci L, Chou K C and Maggiora G M 1991 A heuristic approach to predicting the tertiary structure of bovine somatotropin;Biochemistry 30 4389–4398PubMedCrossRefGoogle Scholar
  8. Cavari B, Funkenstein B and Chen T T 1993 Effect of growth hormone on the growth rate of the gilthead seabream (Sparus aurata) and the use of different constructs for production of transgenic fish;Aquaculture 111 189–197CrossRefGoogle Scholar
  9. Chang Y S, Liu C S, Huang F L and Lo T B 1992 The primary structures of growth hormones of three cyprinoids species: Bighead carp, Silver carp and Grass carp;Gen. Comp. Endocrinol. 87 385–393PubMedCrossRefGoogle Scholar
  10. Chomczynski P and Sacchi N 1987 Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction.Anal. Biochem. 162 156–159PubMedCrossRefGoogle Scholar
  11. Chonders S L 1980Hypophysation of Indian major carps (Agra: Satish book Enterprises) pp 101–108Google Scholar
  12. Corin R E, Guller S, Wu K Y and Sonenberg M 1990 Growth hormone and adipose differentiation: growth hormone induced antimitogenic state in 3T3-F442A preadipose cells;Proc. Natl. Acad. Sci. USA 87 7507–7511PubMedCrossRefGoogle Scholar
  13. Cunningham B C, Ultsch M, De Vos A M, Mulkerrin M G, Clauser K R and Wells J A 1991 Dimerization of the extracellular domain of the human growth hormone receptor by a single hormone molecule;Nature (London) 254 821–825Google Scholar
  14. De Vos A M, Ultsch M and Kossiakoff A A 1992 Human growth hormone and extracellular domain of its receptor: crystal structure of the complex;Science 255 306–312PubMedCrossRefGoogle Scholar
  15. Evans H M and Long J H 1921 The effect of anterior lobe administered intraperitoneally upon growth, maturity and olfactory cycles of rat;Anat. Rec. 21 62–63Google Scholar
  16. Fine M, Edna S, Vashdi D, Daniel V, Lavanon A, Lipshitz O and Gerter A 1993 Recombinant carp growth hormone: Expression, purification and determination of biological activity inin vitro andin vivo;Gen. Comp. Endocrinol. 89 51–61PubMedCrossRefGoogle Scholar
  17. Frohman M A, Dush M K and Martin G R 1988 Rapid amplification of full length cDNA from rare transcripts: amplification using a single gene-specific ologonuclotide primer;Proc. Natl. Acad. Sci. USA 85 8998–9002PubMedCrossRefGoogle Scholar
  18. Guermeur Y, Geourjon C, Gallinari P and Deleage G 1999 Improved performance in protein secondary structure prediction by inhomogenous score combination;Bioinformatics 15 413–421PubMedCrossRefGoogle Scholar
  19. Ghosh S 1996 A novel ligation mediated-PCR based strategy for construction of subtraction libraries from limiting amounts of mRNA;Nucleic Acids Res. 24 795–796PubMedCrossRefGoogle Scholar
  20. Gubler U and Hoffman B J 1983 A simple and very efficient method for generating cDNA libraries;Gene 25 263–269PubMedCrossRefGoogle Scholar
  21. Hara K, Hsu Chen C J and Sonenberg 1978 Recombination of biologically active peptides from a tryptic digest of bovine growth hormone;Biochemistry 17 550–556PubMedCrossRefGoogle Scholar
  22. Hochuli E 1988 Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent;Bio/technology 6 1321–1325CrossRefGoogle Scholar
  23. Kohn W D, Mant C T and Hodges R S 1997 α-Helical protein assembly motifs;J. Biol. Chem. 272 2583–2586PubMedCrossRefGoogle Scholar
  24. Laemmli U K 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4;Nature (London) 227 680–685CrossRefGoogle Scholar
  25. Lemaire C, Wariat S and Panyim S 1994 Giant catfishPangasionodon gigas growth hormone-encoding cDNA: cloning and sequencing by one-sided polymerase chain reaction;Gene 149 271–276PubMedCrossRefGoogle Scholar
  26. Le Gac F, Blaise O, Fostier A, Le Bail P Y, Loir M, Mourot B and Weil C 1993 Growth hormone (GH) and reproduction: A review;Fish Phys. Biochem. 11 219–232CrossRefGoogle Scholar
  27. Li C H and Evans H M 1944 The isolation of pituitary growth hormone;Science 99 183–184PubMedCrossRefGoogle Scholar
  28. Lowry O H, Roseburg N J, Far A L and Randall R J 1951 Protein measurement with folin phenol reagent;J. Biol. Chem. 123 265–275Google Scholar
  29. Mahmoud S S, Moloney M M and Habibs H R 1996 Cloning and sequencing of gold fish growth hormone cDNA;Gen. Comp. Endocrinol. 101 139–144PubMedCrossRefGoogle Scholar
  30. Mathews L, Norstedt G and Palmiter R D 1986 Regulation of insulin like growth factor I gene expression by growth hormone;Proc. Natl. Acad. Sci. USA 83 9343–9347PubMedCrossRefGoogle Scholar
  31. McLean E, Donaldson E M, Teskeredzic E and Souga L M 1993 Growth enhancement following dietary delivery recombinant protein somatotropin to diploid and triploid coho salmon (Oncorhynchus kisutch);Fish Physiol. Biochem. 11 363–369CrossRefGoogle Scholar
  32. Miller W L and Eberhardt N L 1983 Structure and evolution of the growth hormone gene family;Endrocr. Rev. 4 97–130CrossRefGoogle Scholar
  33. Nial H D, Hogan M L and Saver R 1971 Sequence of pituitary and placental lactogenic and growth hormones: Evaluation from a primoridial peptide by gene reduplication;Proc. Natl. Acad. Sci. USA 66 866–870CrossRefGoogle Scholar
  34. Ohara O, Dorit R L and Gilbert W 1989 One-sided polymerase chain reaction: the amplification of cDNA;Proc. Natl. Acad. Sci. USA 86 5673–5677PubMedCrossRefGoogle Scholar
  35. Okayama H and Berg P 1982 High-efficiency cloning of full-length cDNA;Mol. Cell. Biol. 2 161–170PubMedGoogle Scholar
  36. Pandian T J and Marian L A 1994 Problems and prospects of transgenic fish production;Curr. Sci. 66 635–649Google Scholar
  37. Pandian T J, Venugopal T and Koteeswaran R 1999 Problems and prospects of hormones, chromosome and gene manipulations in fish;Curr. Sci. 76 369–386Google Scholar
  38. Ralph R K, Darkin-Rattray S and Schofield P 1990 Growth related protein kinases;BioEssays 12 121–124PubMedCrossRefGoogle Scholar
  39. Reddy P V G V K 1999 Genetic resources of Indian major carps;FAO Fish Tech. Paper 387 18–29Google Scholar
  40. Reiter E O and Rosenfeld 1998 Normal and abnormal growth; inWilliams text book of endocrinology, 9th edition (eds) J D Wilson, D N Foster, H M Kroneberg and P R Larsen (Philadelphia: W B Saunders) pp 1427–1507Google Scholar
  41. Rivolta M N and Wilcox E R 1995 A novel simple methodology to generate substractive cDNA library;Nucleic Acids Res. 23 2565–2566PubMedCrossRefGoogle Scholar
  42. Sakamoto T, McCormick S D and Hirano T 1993 Osmoregulatory actions of growth hormone and its mode of action in salmonids: A review;Fish. Physiol. Biochem. 11 155–164CrossRefGoogle Scholar
  43. Sambrook J, Fritsch E F and Maniatis T 1989Molecular cloning: A laboratory manual (New York: Cold Spring Harbor Laboratory Press)Google Scholar
  44. Sara V R 1991 Insulin like growth factor and fetal growth;Encycl. Hum. Biol. 4 503–511Google Scholar
  45. Schraml P, Shipman R, Stulz P and Ludwig C U 1993 cDNA library construction using a Magnet Assisted Substraction Technology (MAST);Trends Genet. 9 70–71PubMedCrossRefGoogle Scholar
  46. Somers W, Ultsch M, De Vos A M and Kossiakoff A A 1994 The x-ray structure of a growth hormone prolactin receptor complex;Nature (London) 372 478–481CrossRefGoogle Scholar
  47. Tsai H J, Kuo J C, Lou S W and Kuo T T 1994 Growth enhancement of juvenile striped mullet by feeding recombinant yeast containing fish growth hormone;Prog. Fish Cult. 56 7–12CrossRefGoogle Scholar
  48. Van der Kraak G, Rosenblum P M and Peter R E 1990 Growth hormone dependent potentiation of gonodotropin stimulated steroid production by ovarian follicles of the gold fish;Gen. Comp. Endocrinol. 71 233–239CrossRefGoogle Scholar
  49. Venugopal T, Pandian T J and Mathavan S 1998 Growth hormone precursor mRNA complete coding sequence ofLabeo rohita;Genbank submission AF134200Google Scholar
  50. Venugopal T, Anathy V, Pandian T J, Gong G Z and Mathavan S 2002 Molecular cloning of growth hormone encoding cDNA of an Indian major carp,Labeo rohita and its expression inE. coli and Zebrafish;Gen. Comp. Endocrinol. 125 236–247PubMedCrossRefGoogle Scholar
  51. Vestling M, Murphy C, Fenselau C and Chen T T 1991 Disulfide bonds in native and recombinant fish growth hormone;Mol. Mar. Biol. Biotechnol. 1 73–77PubMedGoogle Scholar
  52. Wagner G M, Fargher R C, Brown J C and McKeown B A 1985 Further characterization of growth hormone from the chum salmon(Oncorhynchus keta);Gen. Comp. Endocrinol. 1 27–34CrossRefGoogle Scholar
  53. Wallis 1981 The molecular evolution of pituitary growth hormone, prolactin and placental lactogen: A protein family showing variable rates of evolution;J. Mol. Evol. 17 10–18CrossRefGoogle Scholar
  54. Wallis M 1989 Species specificity and structure-function relationships of growth hormone; inBiotechnology in growth regulation (eds) R B Heap, C G Prosser and G E Lamming (London: Butterworths) pp 3–14Google Scholar
  55. Watahiki M, Yamamoto M and Yamakawa M 1989 Conserved and unique amino acid residues in the domains of the growth hormone. Flounder growth hormone decided from the cDNA sequence has the minimal size in the growth hormone prolactin gene family;J. Biol. Chem. 262 312–316Google Scholar

Copyright information

© Indian Academy of Sciences 2002

Authors and Affiliations

  1. 1.Center for Advanced Studies in Functional Genomics, School of Biological SciencesMadurai Kamaraj UniversityMaduraiIndia

Personalised recommendations