Journal of Biosciences

, Volume 27, Issue 3, pp 261–272 | Cite as

Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

  • T. Venugopal
  • S. Mathavan
  • T. J. PandianEmail author


A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96–98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.


cDNA cloning growth hormone Indian major carps overexpression protein 3-D structure RACE 

Abbreviations used


Growth hormone


guanidium thiocyanate-phenol-chloroform


homology based-rapid amplification of cDNA ends




rapid amplification of cDNA ends


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Copyright information

© Indian Academy of Sciences 2002

Authors and Affiliations

  1. 1.Center for Advanced Studies in Functional Genomics, School of Biological SciencesMadurai Kamaraj UniversityMaduraiIndia

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