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Journal of Biosciences

, Volume 27, Issue 3, pp 243–249 | Cite as

Human placental lipid induces mitogenesis and melanogenesis in B16F10 melanoma cells

  • Shampa Mallick
  • Samir Kumar Mandal
  • Ranjan BhadraEmail author
Article

Abstract

A hydroalcoholic extract of fresh term human placenta was found to be mitogenic as well as melanogenic on B16F10 mouse melanoma in anin vitro culture. The extract, a reservoir of a large number of bioactive molecules, was resolved to get the lipid fraction. Its activity was evaluated on B16F10 mouse melanoma by assessing the change in cellular morphology, growth and melanin induction. The lipid fraction, placental total lipid fraction (PTLF) tested in the study employed doses of 0.01 to 200 μg/ml; optimum growth and melanization accompanied by morphological changes were recorded at 10 and 100 μg/ml respectively. At intermediate doses growth and melanization were found to show a pattern of change over between growth and melanization and finally reached at an inverse relation at the respective optimal dose of response. Compared with defined sphingolipids, C2 ceramide and sphingosine-1-phosphate, the results were mostly corroborative. The duality of biological response of sphingolipids as reported in numerous studies was comparable for the PTLF suggesting that its active component is a sphingolipid and showing its use for pigment recovery in vitiligo.

Keywords

Melanogenesis melanoma mitogenesis morphogenesis placental lipid 

Abbreviations used

C2-cer.

N-acetyl-D-sphingosine

DMEM

Dulbecco’s modified Eagle medium

FBS

fetal bovine serum

MTT

[3-(4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide

PTLF

placental total lipid fraction

SPP

sphingosine-1-phosphate

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Copyright information

© Indian Academy of Sciences 2002

Authors and Affiliations

  • Shampa Mallick
    • 1
  • Samir Kumar Mandal
    • 1
  • Ranjan Bhadra
    • 1
    Email author
  1. 1.Department of Cellular BiochemistryIndian Institute of Chemical BiologyKolkataIndia

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