Journal of Biosciences

, Volume 31, Issue 3, pp 363–368

Aujeszky’s disease virus production in disposable bioreactor

  • I. Slivac
  • V. Gaurina Srček
  • K. RadoŠević
  • I. Kmetič
  • Z. Kniewald
Article

DOI: 10.1007/BF02704109

Cite this article as:
Slivac, I., Srček, V.G., RadoŠević, K. et al. J Biosci (2006) 31: 363. doi:10.1007/BF02704109

Abstract

A novel, disposable-bag bioreactor system that uses wave action for mixing and transferring oxygen was evaluated for BHK 21 C13 cell line growth and Aujeszky’s disease virus (ADV) production. Growth kinetics of BHK 21 C13 cells in the wave bioreactor during 3-day period were determined. At the end of the 3-day culture period and cell density of 1.82 × 106 cells ml-1, the reactor was inoculated with 9 ml of gE- Bartha K-61 strain ADV suspension (105.9 TCID50) with multiplicity of infection (MOI) of 0.01. After a 144 h incubation period, 400 ml of ADV harvest was obtained with titre of 107.0 TCID50 ml−1, which corresponds to 40,000 doses of vaccine against AD. In conclusion, the results obtained with the wave bioreactor using BHK 21 C13 cells showed that this system can be considered as suitable for ADV or BHK 21 C13 cell biomass production.

Keywords

Aujeszky’s disease virus baby hamster kidney cells cell culture disposable bioreactor virus titre 

Abbreviations used:

ADV

Aujeszky’s disease virus

TCID50

tissue culture infectious dose 50.

Copyright information

© Indian Academy of Sciences 2006

Authors and Affiliations

  • I. Slivac
    • 1
  • V. Gaurina Srček
    • 1
  • K. RadoŠević
    • 1
  • I. Kmetič
    • 2
  • Z. Kniewald
    • 1
  1. 1.Laboratory for Cell Culture Technology and BiotransformationsUniversity of ZagrebZagrebCroatia
  2. 2.Laboratory for Toxicology, Faculty of Food Technology and BiotechnologyUniversity of ZagrebZagrebCroatia

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