Journal of Biosciences

, Volume 30, Issue 2, pp 201–211

A non-polyene antifungal antibiotic fromStreptomyces albidoflavus PU 23

  • S. K. Augustine
  • S. P. Bhavsar
  • B. P. Kapadnis
Article

DOI: 10.1007/BF02703700

Cite this article as:
Augustine, S.K., Bhavsar, S.P. & Kapadnis, B.P. J Biosci (2005) 30: 201. doi:10.1007/BF02703700

Abstract

In all 312 actinomycete strains were isolated from water and soil samples from different regions. All these isolates were purified and screened for their antifungal activity against pathogenic fungi. Out of these, 22% of the isolates exhibited activity against fungi. One promising strain,Streptomyces albidoflavus PU 23 with strong antifungal activity against pathogenic fungi was selected for further studies. Antibiotic was extracted and purified from the isolate.Aspergillus spp. was most sensitive to the antibiotic followed by other molds and yeasts. The antibiotic was stable at different temperatures and pH tested and there was no significant loss of the antifungal activity after treatment with various detergents and enzymes. Synergistic effect was observed when the antibiotic was used in combination with hamycin. The antibiotic was fairly stable for a period of 12 months at 4°C. The mode of action of the antibiotic seems to be by binding to the ergosterol present in the fungal cell membrane resulting in the leakage of intracellular material and eventually death of the cell. The structure of the antibiotic was determined by elemental analysis and by ultraviolet (UV), Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR) and liquid chromatography mass spectra (LCMS). The antibiotic was found to be a straight chain polyhydroxy, polyether, non-proteinic compound with a single double bond, indicating a nonpolyene antifungal antibiotic

Keywords

Antibiotic antifungal non-polyene Streptomyces albidoflavus 

Abbreviations used

BJMC

Byramjee Jeejeebhoy Medical College

DEPT

distortionless enhancement by polarization transfer

DSS

dimethyl silapentane sulfonate

FTIR

Fourier transform infrared

LCMS

liquid chromatography mass spectrum

MFC

minimum fungicidal concentration

MIC

minimum inhibitory concentration

MTCC

microbial type culture collection

NCCLS

National Committee for Clinical Laboratory Standards

NCIM

National Collection of Industrial Microorganisms

NMR

nuclear magnetic resonance

NRRL

Northern Regional Research Laboratory

SDA

Sabouraud dextrose agar

SDS

sodium dodecyl sulphate

TLC

thin-layer chromatography

UV

ultraviolet

Copyright information

© Indian Academy of Sciences 2005

Authors and Affiliations

  • S. K. Augustine
    • 1
  • S. P. Bhavsar
    • 1
    • 2
  • B. P. Kapadnis
    • 1
    • 2
  1. 1.PG Department of MicrobiologyAbeda Inamdar Senior CollegePuneIndia
  2. 2.Department of MicrobiologyUniversity of PunePuneIndia

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