Journal of Biosciences

, Volume 19, Issue 1, pp 1–8

Characterization of mitochondrial DNA primase fromSaccharomyces cerevisiae

  • V. Murthy
  • K. Pasupathy
Article

DOI: 10.1007/BF02703462

Cite this article as:
Murthy, V. & Pasupathy, K. J Biosci (1994) 19: 1. doi:10.1007/BF02703462

Abstract

DNA primase from yeast mitochondria was shown to have a molecular weight of 67 kDa by SDS-PAGE and an S value of 5.5. It was shown to have preference for SS mitochondrial DNA especially fragments containing origins of replication, as a template to initiate DNA replication. Further examination of the enzyme showed its possible association with a ribonucleotide moiety essential for enzyme activity.

Keywords

Primase yeast mitochondria 

Copyright information

© Indian Academy of Sciences 1994

Authors and Affiliations

  • V. Murthy
    • 1
  • K. Pasupathy
    • 1
  1. 1.Radiation Biology and Biochemistry DivisionBhabha Atomic Research CentreBombayIndia

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