Journal of Biosciences

, Volume 22, Issue 5, pp 529–536 | Cite as

Trace amounts of enhancing factor/phospholipase A2 in mouse peritoneal exudate cells

  • Abhay R. Redkar
  • Shilpa Kadam
  • Madhav G. Deo
  • Rita Mulherkar
Article
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Revised:
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Abstract

Enhancing factor (EF), a mouse phospholipase A2 (PLA2), has been purified from the small intestines, based on its ability to increase the binding of epidermal growth factor in a radioreceptor assay. EF/PLA2 was found to be localized predominantly in the Paneth cells in the small intestines. Whether mouse intestinal EF/PLA2 is identical/similar to mouse secretory PLA2 was to be determined. Phospholipases are known to play a crucial role in the process of inflammation. This paper reports the presence of trace amounts of EF/PLA2 in the peritoneal exudate cells. Western blot analysis of the acid extracts showed the presence of a 14 kDa immunologically cross-reactive protein. RT-PCR analysis using EF specific primers amplified a ∼700 bp product which was further confirmed to be EF-specific by nested PCR analysis and sequencing. Presence of EF in the peritoneal exudate cells could be a unique mode of transport of growth factor modulator to the site of injury to aid in regeneration/cell proliferation of damaged tissue.

Keywords

Enhancing factor phospholipase A2 inflammation neutrophil Paneth cells EGF 
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Copyright information

© Indian Academy of Sciences 1997

Authors and Affiliations

  • Abhay R. Redkar
    • 1
  • Shilpa Kadam
    • 1
  • Madhav G. Deo
    • 1
  • Rita Mulherkar
    • 1
  1. 1.Laboratory of Genetic EngineeringCancer Research Institute Tata Memorial Centre ParelMumbaiIndia

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