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Journal of Biosciences

, Volume 18, Issue 1, pp 93–101 | Cite as

In vivo andin vitro investigations on rotenoids fromIndigofera tinctoria and their bioefficacy against the larvae ofAnopheles stephensi and adults ofCallosobruchus chinensis

  • R. Kamal
  • M. Mangla
Article

Abstract

Various plant parts ofIndigofera tinctoria L. were collected separately at different growth stages and analysed for their rotenoid content. The total rotenoid content decreased with age; among the plant parts, maximum content was in leaves and minimum in stem. The identity of different rotenoids was confirmed by melting point, mixed melting point, UV and infrared spectral studies, and gas-liquid chromatography. Six rotenoids (deguelin, dehydrodeguelin, rotenol, rotenone, tephrosin and sumatrol) were isolated, identified and quantified invivo.

The static cultures ofIndigofera tinctoria were established from seeds on RT medium, and maintained for a period of six months by frequent subculturings. Only four rotenoids were present in callus cultures; sumatrol and tephrosin were absent. The maximum content was found in eight week old tissue after fresh subculturings and minimum at 2 weeks.

The toxicological studies ofin vivo andin vitro extract against the pulse beetle(Callosobruchus chinensis) and mosquito(Anopheles stephensi) larvae, showed that rotenoids were more effective against mosquito larvae thanCallosobruchus chinensis. Extracts from callus was more effective against both the test animals than that from plant parts.

Keywords

Rotenoids Indigofera tinctoria Anopheles stephensi Callosobruchus chinensis bioefficacy of rotenoids callus culture 

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Copyright information

© Indian Academy of Sciences 1993

Authors and Affiliations

  • R. Kamal
    • 1
  • M. Mangla
    • 1
  1. 1.Laboratory of Plant Physiology and Biochemistry Department of BotanyUniversity of RajasthanJaipurIndia

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