Journal of Biosciences

, Volume 18, Issue 1, pp 93–101 | Cite as

In vivo andin vitro investigations on rotenoids fromIndigofera tinctoria and their bioefficacy against the larvae ofAnopheles stephensi and adults ofCallosobruchus chinensis

  • R. Kamal
  • M. Mangla


Various plant parts ofIndigofera tinctoria L. were collected separately at different growth stages and analysed for their rotenoid content. The total rotenoid content decreased with age; among the plant parts, maximum content was in leaves and minimum in stem. The identity of different rotenoids was confirmed by melting point, mixed melting point, UV and infrared spectral studies, and gas-liquid chromatography. Six rotenoids (deguelin, dehydrodeguelin, rotenol, rotenone, tephrosin and sumatrol) were isolated, identified and quantified invivo.

The static cultures ofIndigofera tinctoria were established from seeds on RT medium, and maintained for a period of six months by frequent subculturings. Only four rotenoids were present in callus cultures; sumatrol and tephrosin were absent. The maximum content was found in eight week old tissue after fresh subculturings and minimum at 2 weeks.

The toxicological studies ofin vivo andin vitro extract against the pulse beetle(Callosobruchus chinensis) and mosquito(Anopheles stephensi) larvae, showed that rotenoids were more effective against mosquito larvae thanCallosobruchus chinensis. Extracts from callus was more effective against both the test animals than that from plant parts.


Rotenoids Indigofera tinctoria Anopheles stephensi Callosobruchus chinensis bioefficacy of rotenoids callus culture 


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  1. Barnes D K and Freyre R H 1966 Recovery of natural insecticide fromTephrosia vogelii II. Toxicological properties of rotenoids extracted from fresh and oven dried leaves;Econ. Bot. 20 368–371Google Scholar
  2. Delfel N E 1966 Thin layer chromatography of rotenoids and related compounds;J. Agric. Food Chem. 14 130–132CrossRefGoogle Scholar
  3. Delfel N E 1973 Rotenone and deguelin quantitation in plant extracts and commercial extracts by gas liquid chromatography;J. Assoc. OffAnalyt. Chem. 56 1343Google Scholar
  4. Delfel N E and Tallent W H 1969 Thin layer densitometric determination of rotenone and deguelin;J. Assoc. Off.Analyt. Chem. 52 182–187Google Scholar
  5. Delfel N E, Tallent W H, Carlson D G and Wolff I A 1970 Distribution of rotenone and deguelin inTephrosia vogelii and separation of rotenoid rich fractions;Agric. Food Chem. 18 385–390CrossRefGoogle Scholar
  6. Finney D G 1952Probit analysis (London: Cambridge University Press)Google Scholar
  7. Fukami H, Takahasi S, Konishi K and Nakajima M 1960 Synthesis of rotenoids I. Synthesis of chromanochromanone and 2 substituted isoflavonones;Bull. Agric. Chem. Soc. Jpn. 24 119–122Google Scholar
  8. Fukui K, Nakayama M and Harano T 1967 A new synthesis if dehydromunduserone;Experientia 23 613–614CrossRefGoogle Scholar
  9. Harborne J B, Mabry T J and Mabry H 1975Theflavonoids (London: Chapman and Hall)Google Scholar
  10. Kamal R and Mangla M 1987 Rotenoids fromIndigofera tinctoria and their bioefficacy against cyclops, the carrier of Dracunculiasis;Pharmazie 42 356PubMedGoogle Scholar
  11. Khanna P and Staba E J 1968 Dioscorea tissue cultures I. Biosynthesis and isolation of diosgenin fromDioscorea deltoidea callus and suspension cells;Lloydia 31 171–179Google Scholar
  12. Kodama T, Yamakawa T and Minoda Y 1980 Rotenoids biosynthesis by tissue culture ofDerris elliptica;Agric. Biol. Chem. 44 2387–2390Google Scholar
  13. Murashige T and Skoog F 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures;Physiol. Plant. 15 473–497CrossRefGoogle Scholar
  14. Steward F C 1969Plant Physiology Vol. 2 (London, New York: Acad. Press)Google Scholar
  15. Roark 1932 Chemically combating insect pests of food stuffs;Indian Eng. Chem. 24 646–648CrossRefGoogle Scholar
  16. Tyagi B K and Das P K 1986 Use of pyrethroid insecticides in controlling vector mosquitoes and their impact on the environment;Proceeding of Symp. Pesti. Resid. Environ. Pollut. 137–149Google Scholar
  17. Worsley R R and Le G 1939 Experimental lemon grass plots in Amani;Bull Imp. Inst. London 37 180–182Google Scholar
  18. World Commission on Environment and Development (WCED) 1987Our common future (New Delhi: Oxford University Press)Google Scholar

Copyright information

© Indian Academy of Sciences 1993

Authors and Affiliations

  • R. Kamal
    • 1
  • M. Mangla
    • 1
  1. 1.Laboratory of Plant Physiology and Biochemistry Department of BotanyUniversity of RajasthanJaipurIndia

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