Journal of Biosciences

, Volume 10, Issue 1, pp 145–151 | Cite as

Mechanism of action of aflatoxin B1

  • R. P. Tiwari
  • T. C. Bhalla
  • S. S. Saini
  • G. Singh
  • D. V. Vadehra


The inhibitory effects of aflatoxin B1 were found to be related to the gram character in procaryotes, used in this study. Ethylene diamine tetra chloroacetic acid (0.05% w/v) or Tween-80 (0.05 % v/v) addition accentuated the aflatoxin B1 growth inhibition inSalmonella typhi andEscherichia coli at different pH values. The inhibition of lipase production was only 5–20 % inPseudomonas fluorescence ca. 25–48% inStaphylococcus aureus andBacillus cereus at different aflatoxin B1 concentrations (4–16μg/ml).However, inhibition of α-amylase induction was complete in1Bacillus megaterium whereas the inhibition was partial inPseudomonas fluorescence (27–40%) at 32μg aflatoxin B1 concentration. An increase in leakage of cell contents and decreased inulin uptake were observed in toxin incubated sheep red blood cell suspension (1 %) with increased aflatoxin B1 concentration


Aflatoxin B1 α-amylase cell content leakage enzyme induction/inhibition lipase procaryotes 

Abbreviations used


Ethylene diamine tetra chloroacetic acid


sheep red blood cell


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  1. Anand, S. R. (1971)Indian J. Exp, Biol.,10,177.Google Scholar
  2. Arai, T., Ito, T. and Koyama, Y. (1967)J. Bacteriol.,93,59.PubMedGoogle Scholar
  3. Asplin, F. D. and Carnagham, R. B. A. (1961)Vet. Res.,73,1215.Google Scholar
  4. Averner, M. and Klein, H. P. (1963)Biochim. Biophys. Acta,77, 510.PubMedCrossRefGoogle Scholar
  5. Bernfeld, P. (1955)Methods Enzymol.,1, 149.CrossRefGoogle Scholar
  6. Beuchat, L. R. and Lechowich, R. V. (1971)Appl Microbiol.,21, 119.PubMedGoogle Scholar
  7. Black, H. S. and Altschul, A. M. (1965)Biochem. Biophys. Res. Commun.,19,661.PubMedCrossRefGoogle Scholar
  8. Blount, W. P. (1961)Turkeys,9,52.Google Scholar
  9. Brown, J. M. M. and Abrams, L. (1965)J. Vet. Res.,32,119.Google Scholar
  10. Burmeister, H. R. and Hesseltine, C. W. (1966)Appl. Microbiol.,14,403.PubMedGoogle Scholar
  11. Butler, W. H. and Barnes, J. M. (1963)Br. J. Cancer,17, 699.PubMedGoogle Scholar
  12. Carnaghan, R. B. A. and Allcroft, R. (1962)Vet. Res.,74, 925.Google Scholar
  13. Childs, V. A. and Legator, M. S. (1966)Life Sci.,5,1053.PubMedCrossRefGoogle Scholar
  14. Clifford, J. I. and Rees, K. R. (1967)Biochem. J.,103,467.PubMedGoogle Scholar
  15. Corcoran, A. C. and Page, I. H. (1939)J. Biol Chem.,121, 601.Google Scholar
  16. Doherty, W. P. and Campbell, T. C. (1972)Res. Commun. Pathol Pharmacol.,3,601.Google Scholar
  17. Gabliks, K. S., Schaeffer, J. W., Friedman, L. and Wogan, G. (1965)J. Bacteriol.,90,720.PubMedGoogle Scholar
  18. Hesseltine, C. W.,Shotwell, O. L., Ellis, J. J. and Stubblefield, R. D. (1966)Bacteriol Rev.,30,795.PubMedGoogle Scholar
  19. Legator, M. S. (1966)Bacteriol. Rev.,30,471.PubMedGoogle Scholar
  20. Leive, L. (1968)J. Biol. Chem.,243,2373.PubMedGoogle Scholar
  21. Lillehoj, E. B. and Ciegler, A. (1968)J. Gen. Microbiol.,54,185.PubMedGoogle Scholar
  22. Lillehoj, E. B. and Ciegler, A. (1970)Can. J. Microbiol.,16,1059.PubMedCrossRefGoogle Scholar
  23. Nabney, J. and Nesbitt, B. F. (1965)Analyst,90,155.CrossRefGoogle Scholar
  24. Nezval, J. and Bosenberg, H. (1970)Arch. Hyg.,154, 143.Google Scholar
  25. Sargeant, K., Sherioan, A., Kelly, J. O. and Carnaghan, R. B. A. (1961)Nature (London),122,1096.CrossRefGoogle Scholar
  26. Shotwell, G. L., Hesseltine, C. W., Stubblefield, R. D. and Sorenson, W. G. (1966)Appl Microbiol.,14,425.PubMedGoogle Scholar
  27. Spensley, P. C. (1963)Endeavour,22, 75.PubMedCrossRefGoogle Scholar
  28. Stark, A. A. (1980)Ann. Rev. Microbiol.,34,235.CrossRefGoogle Scholar
  29. Tiwari, R. P., Dham, C. K.. Gupta, L. K., Saini, S. S., Bhalla, T. C. and Vadehra, D. V. (1984)J. Gen. Appl Microbiol.,30,419.Google Scholar
  30. Tiwari, R. P., Dham, C. K., Bhalla, T. C., Saini, S. S. and Vadehra, D. V. (1985)Appl Environ. Microbiol.,49,904.PubMedGoogle Scholar
  31. Vadehra, D. V. and Harmon, L. G. (1969)J. Appl Bacteriol.,32,147.PubMedGoogle Scholar
  32. Vosdingh, R. A. and Nefe, M. J. C. (1974)Toxicology,2, 107.PubMedCrossRefGoogle Scholar
  33. Wragg, I. B., Rose, V. C. and Legator, M. S. (1967)Proc. Soc. Exp. Biol. Med.,125,1052.PubMedGoogle Scholar

Copyright information

© Indian Academy of Sciences 1986

Authors and Affiliations

  • R. P. Tiwari
    • 1
  • T. C. Bhalla
    • 1
  • S. S. Saini
    • 1
  • G. Singh
    • 1
  • D. V. Vadehra
    • 1
  1. 1.Department of MicrobiologyPanjab UniversityChandigarhIndia

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