Evaluation of shear-induced particle diffusivity in red cell ghosts suspensions
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The shear-induced particle diffusivity in the red blood cell suspensions was evaluated based on the flow model and experimental results in a rectangular flow chamber. The effective diffusivity (De) of solute in the particle suspensions is equal to the stationary diffusivity (Ds) of the solute plus the shear-induced particle diffusivity (Dp). The effective diffusivity (De) of bovine serum albumin (BSA) in the red blood cell (RBC) ghost suspensions was determined under diffusion-limited conditions using a total internal reflection fluorescence (TIRF) method as a function of suspended RBC ghost volume fractions (0.05-0.7) and shear rates (200-1,000 s,-1). The stationary diffusivity (Ds) of BSA in RBC ghost suspensions was calculated by Meredith and Tobias model. Therefore the shear-induced particle diffusivity undergoing laminar shear flow can be evaluated. The shear-induced RBC ghost diffusivity was ranged from 0.35xl0-7 to 21.2xl0-7 cm2/s and it increased with increasing shear rate. Also the shear-induced RBC ghost diffusivity increased as a particle volume fraction increased as well, up to a particle volume fraction of 0.45. However, for RBC ghost volume fractions above 0.45, the shear-induced particle diffusivity decreased with increasing particle volume fraction. The shear-induced particle diffusivity in RBC ghost suspensions is a function of a particle Peclet number (or shear rate) and particle volume fractions. The dimensionless particle diffusivity (Dρ/a2γ) was investigated as a function of particle volume fraction and these results are in good agreement with the literature values.
Key wordsShear-Induced Particle Diffusivity Effective Diffusivity Suspension Red Blood Cell Ghost Particle Motion
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- Bird, R. B., Stewart, W. E. and Lightfoot, E. N., “Transport Phenomena”, John Wiley & Sons Inc. (1960).Google Scholar
- Cha, W., Ph.D. Dissertation, “Red Blood Cell-Augmented Mass Transport of Albumin in Sheared Suspensions to Surfaces,” Illinois Institute of Technology (1993).Google Scholar
- Chin, B. D. and Park, O. O., “Electrorheological Responses of Particulate Suspensions and Emulsions in a Small-Strain Dynamic Shear Flow: Viscoelasticity and Yielding Phenomena,”Korean J. Chem. Eng.,18,54(2001).Google Scholar
- Gauthier, F. J., Goldsmith, H. L. and Mason, S. G., “Flow of Suspensions Through Tubes, X. Liquid Drops as Models of Erythrocytes,”Biorheology,9, 205 (1972).Google Scholar
- Goldsmith, H. L., “Red Cell Motions and Wall Interactions in Tube Flow”,Fed. Proc.,30,1578 (1971).Google Scholar
- Kim, D., Ph.D. Dissertation, “Augmentation of Macromolecular Mass Transport in Sheared Suspensions: The Effective Diffusivity of Gamma Globulin in Red Blood Cell Ghosts Suspensions”, Illinois Institute of Technology (1990).Google Scholar
- Yim, S. S., “A Theoretical and Experimental Study on Cake Filtration with Sedimentation”,Korea J. Chem. Eng.,16,308 (1999).Google Scholar
- Zydney, A. L. and Colton, C. K., “Augmented Solute Transport in the Shear Flow of a Concentrated Suspension,”Physicochemical Hydrodynamics,10, 77 (1988).Google Scholar