, Volume 13, Issue 4, pp 227–234 | Cite as

Myrothecium: A new indoor contaminant?

  • Estelle Levetin
  • Richard Shaughnessy
Original Articles


In the natural environmentMyrothecium species occur as soil or leaf surface saprobes or as weak plant pathogens. In addition, some species ofMyrothecium are known to produce trichothecene mycotoxins. During a previous aerobiological investigation at two Las Vegas elementary schools,Myrothecium conidia were found to be the second most abundant spore type identified indoors from Burkard personal spore trap samples. The present study was undertaken to re-examine the schools to locate the source ofMyrothecium spores and to examine the ability ofMyrothecium to grow on indoor substrates. There were no obvious signs of contamination in the classrooms; however,Myrothecium spores occurred on about 30% of the Burkard samples. Two colonies ofMyrothecium were identified from subcultures of the Andersen samples, and three colonies were identified from carpet dust samples. Culture studies showed that a strain ofMyrothecium cinctum was able to grow on various culture media as well as on various indoor substrates including paper, cardboard, wallpaper, ceiling tiles, dry wall, carpets and cotton rug. Although there was no attempt to estimate any human health risks, these investigations are believed to be the first to document abundantMyrothecium spores from indoor air samples.


Myrothecium Fungal spores Mycotoxins Indoor air 


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  1. Bean GA, Jarvis BB, Aboul-Nasr MB. A biological assay for the detection ofMyrothecium spp. produced macrocyclic trichothecens. Mycopathologia 1992;119(3):175–80.CrossRefGoogle Scholar
  2. Bergman F, Yagen B, Jarvis BB. The toxicity of macrocyclic trichothecenes administered directly into the rat brain. Toxicon 1992;30(10):1291–4.CrossRefGoogle Scholar
  3. Buttner MP, Cole LEM, Stetzenbach LD. Monitoring for Airborne Fungi at Two Elementary Schools in Las Vegas, Nevada. Report submitted for EPA School Evaluation Project. 1994.Google Scholar
  4. Ellis NB, Ellis JP. Microfungi on Land Plants—An Identification Handbook. New York: Macmillan Publishing, 1985.Google Scholar
  5. Fitt BDL, McCartney HA, Walklate PJ. The role of rain in dispersal of pathogen inoculum. Annu Rev Phytopathol 1989;27:241–70.CrossRefGoogle Scholar
  6. Flannigan B, Miller JD. Health implications of fungi in indoor environments—an overview. In: Samson RA, Flannigan B, Flannigan ME, Verhoeff AP, Adan OCG, Hoekstra ES, editors. Health Implications of Fungi in Indoor Environments. Amsterdam: Elsevier, 1994:3–28.Google Scholar
  7. Griffin, DH. Fungal Physiology, 2nd ed., New York, Wiley-Liss, 1994.Google Scholar
  8. Hocking AD, Pitt JI. Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods. Appl Environ Microbiol 1980;39(3):488–92.Google Scholar
  9. Huber L, Gillespie TJ. Modeling leaf wetness in relation to plant disease epidemiology. Annu Rev Phytopathol 1992;30:553–77.CrossRefGoogle Scholar
  10. Levetin E. Indoor Air Report for Bioaerosols in Two Las Vegas Elementary Schools. Report submitted for EPA School Evaluation Project. 1995.Google Scholar
  11. Levetin E, Shaughnessy R, Stetzenbach L. A case study of airborne fungi: why viable sampling is not enough. J Allergy Clin Immunol 1996;97(1):222.CrossRefGoogle Scholar
  12. Moss MO. Mycotoxins. Mycol Res 1996;100:513–23.CrossRefGoogle Scholar
  13. Mueller E, Loeffler W. Mycology. Stuttgart: Georg Thieme Publishers, 1976.Google Scholar
  14. Ueno Y. Trichothecenes—Chemical, Biological and Toxicological Aspects. Amsterdam: Elsevier, 1983.Google Scholar
  15. Watanabe T. Pictorial Atlas of Soil and Seed Fungi. Boca Raton (FL): CRC Press, 1994.Google Scholar
  16. Yang CS, Johanning E. Airborne Fungi and Mycotoxins. In: Hurst CJ, Knudsen GR, McInerney MJ, Stetzenbach LD, Walter MV, editors. Manual of Environmental Microbiology. Washington DC: ASM Press, 1997:651–660.Google Scholar

Copyright information

© Published by Elsevier Science Ireland Ltd 1997

Authors and Affiliations

  • Estelle Levetin
    • 1
  • Richard Shaughnessy
    • 2
  1. 1.Faculty of Biological Science, Department of Chemical EngineeringThe University of TulsaTulsaUSA
  2. 2.Department of Chemical Engineeringthe University of TulsaTulsaUSA

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