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Chromatographia

, Volume 24, Issue 1, pp 433–438 | Cite as

Separation of polypeptide antibiotics by reversed-phase high-performance liquid chromatography

  • H. -P. Fiedler
  • T. Hörner
  • A. Wörn
Article

Summary

The influence of different reversed-phase packings and the addition of acidic modifiers to the mobile phase was observed on the separation of basic and neutral polypeptide antibiotics by gradient elution. A dependence of pore size, coverage, reaction type and endcapping of the packings was not observed. Nevertheless, not all reversed-phase packings were suitable for the separation of polypeptides, especially of basic molecules. The addition of phosphoric or perchloric acid to the mobile phase prevented adsorption of the basic polypeptide antibiotics on the stationary phase.

Key Words

Column liquid chromatography Adsorption effects Polypeptide antibiotics Quantitative determination 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • H. -P. Fiedler
    • 1
  • T. Hörner
    • 1
  • A. Wörn
    • 1
  1. 1.Institut für Biologie 11, Lehrstuhl Mikrobiologie 1Universität TübingenTübingenFRG

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