Chromatographia

, Volume 41, Issue 5–6, pp 539–543 | Cite as

Temperature-programmed high performance liquid chromatography separation of mono- and divinyl chlorophyll forms from marine phytoplankton

  • K. Van Lenning
  • J. L. Garrido
  • J. Aristegui
  • M. Zapata
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Summary

Divinyl chlorophyll a (a2) and divinyl chlorophyll b (b2) are chemotaxonomic marker pigments for the marine prochlorophytes, and can be used to study their distribution in marine samples. In this paper we report a baseline resolution of mono- and divinyl forms of chlorophylls a and b employing polymeric ODS stationary phases at sub-ambient temperatures. The simultaneous resolution of mono- and divinyl forms of chlorophylls a and b, chlorophylls c1, c2, c3, Mg 3,8-divinylphaeoporphyrin a5 monomethyl ester (MgDVP), and phytol-substituted chlorophylls c was achieved when a temperature step gradient was employed during the analysis. An example is given of the utility of the protocol in oceanic field samples.

Key Words

Column liquid chromatography Divinyl chlorophyll a and b (chl a2 and chl b2Polymeric ODS phases Prochlorophytes Temperature step gradients 

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References

  1. [1]
    S. W. Chisholm, R. J. Olson, E. R. Zettler, R. Goericke, J. B. Waterbury, Nature334, 340 (1988).CrossRefGoogle Scholar
  2. [2]
    S. W. Chisholm, S. L. Frankel, R. Goericke, R. J. Olson, B. Palenik, J. B. Waterbury, L. West-Johnrud, E. R. Zettler, Arch. Microbiol.157, 297 (1992).CrossRefGoogle Scholar
  3. [3]
    R. Goericke, D. J. Repeta, Limnol. Oceanogr.37, 425 (1992).CrossRefGoogle Scholar
  4. [4]
    W. W. Gieskes, G. W. Kraay, Limnol. Oceanogr.28, 757 (1983).CrossRefGoogle Scholar
  5. [5]
    R. F. C. Mantoura, C. A. Llewellyn, Anal. Chim. Acta151, 297 (1983).CrossRefGoogle Scholar
  6. [6]
    S. W. Wright, S. W. Jeffrey, R. F. C. Mantoura, C. A. Llewellyn, T. Bjørnland, D. Repeta, N. Welschmeyer, Mar. Ecol. Prog. Ser.77, 183 (1991).CrossRefGoogle Scholar
  7. [7]
    G. W. Kraay, M. Zapata, M. J. W. Veldhuis, J. Phycol.28, 708 (1992).CrossRefGoogle Scholar
  8. [8]
    R. Goericke, D. J. Repeta, Mar. Ecol. Prog. Ser.101, 307 (1993).CrossRefGoogle Scholar
  9. [9]
    G. B. McManus, R. Dawson, Mar. Ecol. Prog. Ser.113, 199 (1994).CrossRefGoogle Scholar
  10. [10]
    M. J. W. Veldhuis, G. W. Kraay, Neth. J. Sea Res.31, 135 (1993).CrossRefGoogle Scholar
  11. [11]
    L. Van Heukelen, A. J. Lewitus, T. M. Kana, N. E. Craft, J. Phycol.28, 867 (1992).CrossRefGoogle Scholar
  12. [12]
    J. L. Garrido, M. Zapata, Chromatographia35, 543 (1993).CrossRefGoogle Scholar
  13. [13]
    J. L. Garrido, M. Zapata, J. High Resolut. Chromatogr.16, 229 (1993).CrossRefGoogle Scholar
  14. [14]
    L. C. Sander, S. A. Wise, Anal. Chem.61, 1749 (1989).CrossRefGoogle Scholar
  15. [15]
    L. Van Heukelen, A. J. Lewitus, T. M. Kana, N. E. Craft, Mar. Ecol. Prog. Ser.114, 303 (1994).CrossRefGoogle Scholar
  16. [16]
    J. L. Garrido, M. Zapata, S. Mañiz, J. Phycol. (in press).Google Scholar
  17. [17]
    M. J. W. Veldhuis, G. W. Kraay, Mar. Ecol. Prog. Ser.68, 121 (1990).CrossRefGoogle Scholar
  18. [18]
    M. Zapata, J. L. Garrido, Chromatographia31, 589 (1991).CrossRefGoogle Scholar
  19. [19]
    M. Zapata, A. M. Ayala, J. M. Franco, J. L. Garrido, Chromatographia23, 26 (1987).CrossRefGoogle Scholar
  20. [20]
    S. Liaaen-Jensen, Pure Appl. Chem.61, 369 (1989).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1995

Authors and Affiliations

  • K. Van Lenning
    • 1
  • J. L. Garrido
    • 2
  • J. Aristegui
    • 1
  • M. Zapata
    • 3
  1. 1.Facultad de Ciencias del MarUniversidad de Las Palmas de Gran CanariaLas PalmasSpain
  2. 2.Instituto de InvestigacionesCSICVigoSpain
  3. 3.Centro de Investigaciones MariñasConselleria de PescaVilagarcia de ArouseSpain

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