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Chromatographia

, Volume 19, Issue 1, pp 246–250 | Cite as

Precolumn switching in HPLC quantitation of small amounts of microbial metabolites as an alternative method to off-line extraction

  • H. -P. Fiedler
  • H. P. Reuschenbach
Article

Summary

On-line sample enrichment by precolumn switching is compared with off-line extraction by the Extrelut technique. Both methods showed nearly the same product recovery in routine HPLC analysis. Precolumn switching is distinguished by injection of biological samples without pretreatment, high precision and is eminently suited for automated routine analysis. The system has been applied to the quantitative determination of phosphorylated lactones in the fermentation broth ofStreptomyces antibioticus TÜ 1718.

Key Words

High-performance liquid chromatography Off-line sample enrichment On-line sample enrichment Precolumn switching Quantitative determination Fermentation samples 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgeselischaft mbH 1984

Authors and Affiliations

  • H. -P. Fiedler
    • 1
  • H. P. Reuschenbach
    • 1
  1. 1.Institut für Biologie II, Lehrstuhl Mikrobiologie ITübingenFRG

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