Biological Trace Element Research

, Volume 116, Issue 1, pp 91–102 | Cite as

Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs

  • Shunyi Qin
  • Jianzhong Gao
  • Kehe Huang
Article

Abstract

Thirty-two wether lambs of Tan sheep were randomly assigned into four dietary treatment groups (eight per group) for an 8-wk study and then fed a basal diet deficient in Se (0.06 mg/kg) or diets supplemented to provide 0.10 mg/kg Se from sodium selenite, selenized yeast, and selenium-enriched probiotics, respectively. Blood samples were collected at d 0, 28, and 56 of the experiment and tissue samples were collected at experiment termination. Tissue and blood Se concentrations, blood glutathione peroxidase (GSH-Px) activities, and plasma interleukin levels were analyzed. The results showed that the concentrations of Se in the kidney, liver, and muscle increased in all of the supplemented groups (p<0.01) compared with the control group. However, the Se concentrations in the kidney, liver, and muscle in the groups supplemented with Se yeast and Se-enriched probiotics were higher than those in the group supplemented with sodium selenite (p<0.01). The activities of GSH-Px and the concentrations of Se in blood also increased in all of the supplemented groups during the period of supplementation (p<0.01) compared with the control group. The activities of GSH-Px and the concentrations of Se in the whole blood of the lambs fed with selenized yeast and Se-enriched probiotics were higher than those of lambs fed with sodium selenite (p<0.01 or p<0.05). The concentrations of interleukin-1 and interleukin-2 in plasma significantly increased in all of the supplemented groups during the entire period of experiment (p<0.01) compared with the control group, but had no significant differences among all of the supplemented groups. In conclusion, a diet supplemented with Se for finishing lambs was able to increase the concentrations of Se in tissue and blood, activities of GSH-Px in blood, and levels of interleukins in plasma. Organic Se sources (selenized yeast and Se-enriched probiotics) were more effective than the inorganic Se source (sodium selenite) in increasing tissue and blood Se concentrations and blood GSH-Px activities of lambs. However, there were no significant differences in plasma interleukin levels of lambs between organic and inorganic Se sources.

Index Entries

Organic selenium inorganic selenium tissue selenium concentration GSH-Px interleukins lambs 

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References

  1. 1.
    E. Beytut, F. Karatas, and E. Beytut, Lambs with white muscle disease and selenium content of soil and meadow hay in the region of Kars, Turkey, Vet. J. 163, 214–217 (2002).PubMedCrossRefGoogle Scholar
  2. 2.
    J. R. Arthur, The glutathione peroxidases, Cell. Mol. Life Sci. 57, 1825–1835 (2000).PubMedCrossRefGoogle Scholar
  3. 3.
    R. G. Hemingway, The influences of dietary intakes and supplementation with selenium and vitamin E on reproduction diseases and reproductive efficiency in cattle and sheep, Vet. Res. Commun. 27, 159–174 (2003).PubMedCrossRefGoogle Scholar
  4. 4.
    R. C. McKenzie, T. S. Rafferty, and G. J. Beckett, Selenium: an essential element for immune function, Immunol. Today 19, 342–345 (1998).PubMedCrossRefGoogle Scholar
  5. 5.
    K. Huang, and S. Yang, Inhibitory effect of selenium on Cryptosporidium parvum Infection in vitro and in vivo, Biol. Trace Element Res. 90, 261–272 (2002).CrossRefGoogle Scholar
  6. 6.
    R. F. Grimble, Modification of inflammatory aspects of immune function by nutrients, Nutr. Res. 18, 1297–1317 (1998).CrossRefGoogle Scholar
  7. 7.
    R. D. Wang, C. S. Wang, Z. H. Feng, and Y. Luo, Investigation on the effect of selenium on T lymphocyte proliferation and its mechanisms, Acta Univ. Med. Tongji (China) 12(2), 33–38 (1992).Google Scholar
  8. 8.
    N. Safir, A. Wendel, R. Saile, and L. Chabraoui, The effect of selenium on immune functions of J774.1 cells, Clin. Chem. Lab. Med. 41, 1005–1011 (2003).PubMedCrossRefGoogle Scholar
  9. 9.
    J. Gao, Effects of different selenium sources on immune function and antioxidative capacity and their mechanisms in weaned piglets and lamb, PhD thesis, Nanjing Agricultural University, Nanjing, China (2005).Google Scholar
  10. 10.
    G. Y. Wang, R. H. Zhou, S. Z. Sun, T. A. Yin, and G. Q. Yang, Methods of fluorometric determination of trace amount of selenium in biological materials, water and soil 1. Determination of selenium in hair, blood, urine and other organs, Acta Nutr. Sin. (China) 7(1), 39–45 (1985).Google Scholar
  11. 11.
    W. J. Hartley and A. B. Grant, A review of selenium responsive diseases of New Zealand livestock, Fed. Proc. 20, 679–688 (1961).PubMedGoogle Scholar
  12. 12.
    J. J. Wichtel, A. L. Craigie, D. A. Freeman, H. Varela-Alvarez, and N. B. Williamson, Effect of selenium and iodine supplementation on growth rate, thyroid and somatotropic function in dairy calves at pasture, J. Dairy. Sci. 79, 1865–1872 (1996).PubMedGoogle Scholar
  13. 13.
    W. P. Weiss, V. F. Colenbrander, M. D. Cunningham, and C. J. Callahan, Selenium/vitamin E: role of disease prevention and weight gain of neonatal calves, J. Dairy Sci. 66, 1101–1107 (1983).PubMedGoogle Scholar
  14. 14.
    S. A. Gunter, P. A. Beck, and J. M. Phillips, Effects of supplementary selenium source on the performance and blood measurements in beef cows and their calves, J. Anim. Sci. 81, 856–864 (2003).PubMedGoogle Scholar
  15. 15.
    C. Daun and B. Akesson, Glutathione peroxidase activity and content of total and soluble selenium in five bovine and porcine organs used in meat production, Meat. Sci. 66, 801–807 (2004).CrossRefGoogle Scholar
  16. 16.
    L. A. Cristaldi, L. R. McDowell, C. D. Buergelt, and P. A. Davis, Tolerance of inorganic selenium in wether sheep, Small Ruminant Res. 56, 205–213 (2004).CrossRefGoogle Scholar
  17. 17.
    C. F. Ehlig, D. E. Hogue, W. H. Allaway, and D. J. Hamm, Fate of selenium from selenite or selenomethionine with or without vitamin E in lambs, J. Nutr. 92, 121–126 (1967).PubMedGoogle Scholar
  18. 18.
    J. B. J. Van Ryssen, J. T. Deagen, M. A. Beilstein, and P. D. Whanger, Comparative metabolism of organic and inorganic selenium by sheep, J. Agric. Food Chem. 37, 1358–1363 (1989).CrossRefGoogle Scholar
  19. 19.
    B. Shi and J. E. Spallholz, Selenium from beef is highly bioavailable as assessed by liver glutathione peroxidase activity (EC 1.11.1.9) and tissue selenium, J. Nutr. 72, 873–881 (1994).CrossRefGoogle Scholar
  20. 20.
    J. W. G. Nicholson, R. E. McGzueen, and R. S. Bush, Response of growing cattle to supplementation with organically bound or inorganic sources of selenium or yeast cultures. Can. J. Anim. Sci. 71, 803–811 (1991).CrossRefGoogle Scholar
  21. 21.
    S. O. Knowles, N. D. Grace, K. Wurms, and J. Lee, Significance of amount and form of dietary selenium on blood, milk and casein selenium concentrations in grazing cows J. Dairy Sci. 82, 429–437 (1999).PubMedCrossRefGoogle Scholar
  22. 22.
    P. H. Anderson, S. Berrett, and D. S. P. Patterson, The biological selenium status of livestock in Britain as indicated by sheep erythrocyte glutathione peroxidase activity, Vet. Rec. 104, 235–238 (1979).PubMedGoogle Scholar
  23. 23.
    J. J. Wichtel, A review of selenium deficiency in grazing ruminants, NZ Vet. J. 46(2), 47–58 (1998).Google Scholar
  24. 24.
    F. T. Awadeh, R. L. Kincaid, and K. A. Johnson, Effect of level and source of dietary selenium on concentrations of thyroid hormones and immunoglobulins in beef cows and calves, J. Anim. Sci. 76, 1204–1215 (1998).PubMedGoogle Scholar
  25. 25.
    M. J. Rock, R. L. Kincaida, and G. E. Carstens, Effects of prenatal source and level of dietary selenium on passive immunity and thermometabolism of newborn lambs, Small Ruminant Res. 40, 129–138 (2001).CrossRefGoogle Scholar
  26. 26.
    D. C. Mahan, T. R. Cline, and B. Richert, Effects of dietary levels of selenium-enriched yeast and sodium selenite as selenium sources fed to growing-finishing pigs on performance, tissue selenium, serum glutathione peroxidase activity, carcass characteristics, and loin quality, J. Anim. Sci. 77, 2172–2179 (1999).PubMedGoogle Scholar
  27. 27.
    G. N. Schrauzer, The nutritional significance, metabolism and toxicology of selenomethionine, Adv. Food Nutr. Res. 47, 73–112 (2003).PubMedCrossRefGoogle Scholar
  28. 28.
    J. A. Butler, M. A. Beilstein, and P. D. Whanger, Influence of dietary methionine on the metabolism of selenomethionine in rats, J. Nutr. 119, 1001–1009 (1989).PubMedGoogle Scholar
  29. 29.
    C. A. Swanson, B. H. Patterson, O. A. Levander, et al., [74Se] selenomethionine metabolism: a kinetic model, Am. J. Clin. Nutr. 54, 917–926 (1991).PubMedGoogle Scholar
  30. 30.
    K. M. Koenig, L. M. Rode, R. D. Cohen, and W. T. Buckley, Effects of diet and chemical form of selenium on selenium metabolism in sheep, J. Anim. Sci. 75, 817–827 (1997).PubMedGoogle Scholar
  31. 31.
    F. B. Cousins and I. M. Cairney, Some aspects of selenium metabolism in sheep, Aust. J. Agric. Res. 12, 927–943 (1961).CrossRefGoogle Scholar
  32. 32.
    V. J. Johnson, M. Tsunoda, and R. P. Sharma, Increased production of proinflammatory cytokines by murine macrophages following oral exposure to sodium selenite but not to seleno-l-methionine, Arch. Environ. Contam. Toxicol. 39, 243–250 (2000).PubMedCrossRefGoogle Scholar
  33. 33.
    R. L. Brown, R. L. Griffith, F. W. Ruscetti, and H. Rabin, Modulation of interleukin 2 release from a primate lymphoid cell line in serum-free and serum-containing media, Cell. Immunol. 92, 14–21 (1985).PubMedCrossRefGoogle Scholar
  34. 34.
    L. Kiremidjian-schumacher, M. Roy, H. I. Wishe, M. W. Cohen, and G. Stotzky, Selenium and immune cell functions. I. Effect on lymphocyte proliferation and production of interleukin 1 and interleukin 2, Proc. Soc. Exp. Biol. Med. 193, 136–142 (1990).PubMedGoogle Scholar
  35. 35.
    L. D. Koller, J. H. Exon, P. A. Talcott, C. A. Osborne, and G. M. Henningsen, Immune responses in rats supplemented with selenium, Clin. Exp. Immunol. 63, 570–576 (1986).PubMedGoogle Scholar
  36. 36.
    E. S. Aziz and P. H. Klesius, The effect of selenium deficiency in goats on lymphocyte production of leukocyte migration inhibitory factor, Vet. Immunol. Immunopathol. 10, 381–390 (1985).PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc 2007

Authors and Affiliations

  • Shunyi Qin
    • 1
  • Jianzhong Gao
    • 2
  • Kehe Huang
    • 1
  1. 1.College of Veterinary MedicineNanjing Agricultural UniversityNanjingChina
  2. 2.College of Life ScienceShanghai Fisheries UniversityShanghaiChina

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