Advertisement

Ichthyological Research

, Volume 47, Issue 2, pp 157–161 | Cite as

Are morphological characters distinctive enough to discriminate between two species of freshwater eels,anguilla celebesensis andA. interioris?

  • Jun Aoyama
  • Shun Watanabe
  • Satoshi Ishikawa
  • Mutsumi Nishida
  • Katsumi Tsukamoto
Article

Abstract

To test the reliability of morphological keys for identifying two freshwater eel species,Anguilla celebesensis andA. interioris, mitochondrial DNA sequences were analyzed. Of eleven specimens examined, five were from Sulawesi and six from New Guinea. Specimens were morphologically identified as tenA. celebesensis and oneA. interioris. However, mitochondrial DNA sequence analysis indicated the existence of two genetically distinct groups, which corresponded exactly to the sampling localities. This contradiction might be attributable to inadequate descriptions of the anguillid species, for which intra-specific variations have been significantly underestimated. New approaches such as molecular analysis, are necessary for species’ identification of freshwater eels.

Key words

Anguilla species identification morphology mtDNA New Guinea 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. Aoyama, J. and K. Tsukamoto. 1997. Evolution of the freshwater eels. Naturwissenschaften, 84: 17–21.CrossRefPubMedGoogle Scholar
  2. Aoyama, J., T. Kobayashi and K. Tsukamoto. 1996. Phylogeny of eels suggested by mitochondrial sequences. Nippon Suisan Gakkaishi, 62: 370–375. (In Japanese.)CrossRefGoogle Scholar
  3. Castle, P. H. J. and G. R. Williamson. 1974. On the validity of the fresh water eel speciesAnguilla ancestralis Ege, from Celebes. Copeia, 2: 569–570.CrossRefGoogle Scholar
  4. Ege, V. 1939. A revision of the genusAnguilla shaw, a systematic, phylogenetic and geographical study. Dana Rep., 16: 1–256.Google Scholar
  5. Felsenstein, J. 1993. PHYLIP: Phylogeny inference package, version 3.5. University of Washington, Washington.Google Scholar
  6. Ishikawa, S., 1998. Molecular study on the population structure ofAnguilla marmorata. Unpubl. Doctral thesis, Univ. of Tokyo, Tokyo. 156pp. (In Japanese).Google Scholar
  7. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol., 16: 111–120.CrossRefPubMedGoogle Scholar
  8. Koehn, R. K. and G. C. Williams. 1978. Genetic differentiation without isolation in the American eel,Anguilla rostrata II. Temporal stability of geographic patterns. Evolution, 32: 624–637.CrossRefGoogle Scholar
  9. Miya, M. and M. Nishida 1996. Molecular phylogenetic prespective on the evolution of the deep-sea fish genusCyclothone (Stomüformes: Gonostomatidae). Icthyol. Res. 43: 375–398.CrossRefGoogle Scholar
  10. Palumbi, S., A. Martin, S. Romano, W. O. McMillan, L. Stice, and G. Grabowski. 1991. The simple fool’s guide to PCR Ver. 2.0. University of Hawaii. 40 pp.Google Scholar
  11. Springer, V. G. 1982. Pacific plate biogeography with special reference to shorefishes. Smithsonian Contrib. Zool., 367: 1–182.CrossRefGoogle Scholar
  12. Stepien, C. A. and T. D. Kocher. 1997. Molecules and morphology in studies of fish evolution. Pages 1–11,in T. D. Kocher and C. A. Stepien, eds. Molecular systematics of fishes. Academic Press, San Diego.CrossRefGoogle Scholar
  13. Swofford, D. L. 1991. PAUP: Phylogenetic analysis using parsimony, Ver. 3.0. Illinois natural history survey. Illinois.Google Scholar
  14. Williams, G. C., R. K. Koehn, and J. B. Mitton. 1973. Genetic differentiation without isolation in the American eel,Anguilla rostrata, Evolution 27: 192–204.CrossRefGoogle Scholar

Copyright information

© The Ichthyological Society of Japan 2000

Authors and Affiliations

  • Jun Aoyama
    • 1
  • Shun Watanabe
    • 1
  • Satoshi Ishikawa
    • 1
  • Mutsumi Nishida
    • 2
  • Katsumi Tsukamoto
    • 1
  1. 1.Ocean Research InstituteUniversity of TokyoTokyoJapan
  2. 2.Department of Marine BioscienceFukui Prefectural UniversityFukuiJapan

Personalised recommendations