Journal of the American Oil Chemists’ Society

, Volume 58, Issue 12, pp A938–A940 | Cite as

Replacement of benzene as a solvent for aflatoxin standards

  • J. Velasco


Cyclohexane, heptane and toluene were evaluated for possible replacement of benzene in the preparation of aflatoxin standard solutions. The results of a 6-month study showed that these solvents can be substituted for benzene, provided they are not unduly exposed to light. The superiority of benzene as a solvent for aflatoxin standards was clearly demonstrated in a test in which these solvent systems containing 5μg B1 /mL, were exposed to daily lighting in the laboratory for 3 months. Complete photodegradation of aflatoxin B1 occurred in the cyclohexane, heptane and toluene systems whereas only a 15% B1 decrease occurred in the benzene system.


Cyclohexane Aflatoxin Heptane OSHA Benzene System 
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  1. 1.
    Stoloff, L., A.C. Beckwith and M.E. Cushmac, J. Assoc. Off. Anal. Chem. 51:65 (1968).Google Scholar
  2. 2.
    Robertson, J.A., W.A. Pons, Jr., and L.A. Goldblatt Ibid. 53:299(1970).Google Scholar

Copyright information

© American Oil Chemists’ Society 1981

Authors and Affiliations

  • J. Velasco
    • 1
  1. 1.U.S. Department of AgricultureARS, Seed Research LaboratoryBeltsville

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