Jojoba oil analysis by high pressure liquid chromatography and gas chromatography/mass spectrometry

  • Gayland F. Spencer
  • Ronald D. Plattner
  • Thomas Miwa


Two analytical procedures for determining com-positions of jojoba liquid wax esters are described and compared. One, the more tedious, involves separation of wax ester homologs by high pressure liquid chro-matography followed by determination of the acid and alcohol moieties from each homolog. The second allows rapid determination of wax ester composition by gas Chromatographic separation of hydrogenated jojoba wax esters according to chain length, followed immediately by ancillary mass spectrometric identifi-cation of the acid and alcohol moieties. Double bonds in the alkyl chains in jojoba liquid waxes were almost exclusively (98%) ω-9, when examined by gas chro-matography/mass spectrometry (GC/MS) and ozonolysis/GC/MS.


High Pressure Liquid Chromatography Jojoba Double Bond Position Alcohol Moiety High Pressure Liquid Chro 
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Copyright information

© American Oil Chemists’ Society 1977

Authors and Affiliations

  • Gayland F. Spencer
    • 1
  • Ronald D. Plattner
    • 1
  • Thomas Miwa
    • 1
  1. 1.Northern Regional Research Center, ARS, USDAPeoria

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