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Ichthyological Research

, Volume 47, Issue 1, pp 43–50 | Cite as

Phylogenetic relationships of the Japanese minnows,Pseudorasbora (Cyprinidae), as inferred from mitochondrial 16S rRNA gene sequences

  • Katsutoshi Watanabe
  • Kei'ichiroh Iguchi
  • Kazumi Hosoya
  • Mutsumi Nishida
Article

Abstract

The phylogenetic relationships among threePseudorasbora fishes (Cyprinidae, Sarcocheiichthyinae) occurring in Japan (P. parva, P. pumila pumila andP. pumila subsp. sensu Nakamura [1963]) were inferred from nucleotide sequences of the mitochondrial 16S rRNA gene. The sequences. of 1240 bp, were determined and compared for 22 specimens from 2–8 populations for each taxon, with a singlePungtungia herzi specimen as an outgroup. A total of 171 sites (13.8%) were variable among the specimens, but only 0–2 sites within each population. The phylogenetic relationships estimated by neighbor-joining, maximum-parsimony and maximum-likelihood methods confirmed a sister relationship between the twoP. pumila subspecies, with a high level of confidence. However, their genetic distinction from each other (4.1±0.4SD % sequence difference on average) was at a level similar to that between them andP. parva (5.9±0.5%). The geographic distribution of the twoP. pumila subspecies, which are separated by the Fossa Magna region, suggests that the genetic divergence of the two subspecies originated from a vicariant process separating the freshwater ichthyofaunas of eastern and western Honshu.Pseudorasbora parva populations were divided into two genetic groups (1.8±0.2% sequence difference), one group comprising continental and part of the Japanese populations, and the other the remaining Japanese populations. This suggests that at least two genetically divergent lineages had been originally distributed in Japan, but a strong possibility remains that the present situation has resulted from artificial transplantation.

Key words

Pseudorasbora Cyprinidae mitochondrial DNA 16S rRNA molecular phylogeny 

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Copyright information

© The Ichthyological Society of Japan 2000

Authors and Affiliations

  • Katsutoshi Watanabe
    • 1
  • Kei'ichiroh Iguchi
    • 2
  • Kazumi Hosoya
    • 2
  • Mutsumi Nishida
    • 1
  1. 1.Department of Marine BioscienceFukui Prefectural UniversityFukuiJapan
  2. 2.National Research Institute of Fisheries ScienceNaganoJapan

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