Nuclear DNA content of some important plant species
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Nuclear DNA contents of more than 100 important plant species were measured by flow cytometry of isolated nuclei stained with propidium iodide.Arabidopsis exhibits developmentally regulated multiploidy and has a 2C nuclear DNA content of 0.30 pg (145 Mbp/1C), twice the value usually cited. The 2C value for rice is only about three times that ofArabidopsis. Tomato has a 2C value of about 2.0 pg, larger than commonly cited. This survey identified several horticultural crops in a variety of families with genomes only two or three times as large asArabidopsis; these include several fruit trees (a pricot, cherry, mango, orange, papaya, and peach). The small genome sizes of rice and the horticultural plants should facilitate molecular studies of these crops.
Key wordsGenome size flow cytometry propidium iodide Arabidopsis rice tomato
DNA content of the unreplicated haploid chromosome complement
chicken red blood cells
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- Arumuganathan, K. and E.D. Earle 1991. Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol. Biol. Reporter 9:221–231.Google Scholar
- Bennett, M.D. 1985. Intraspecific variation in DNA amount and the nucleotypic dimension in plant genetics. In:Plant Genetics (ed. M. Freeling), pp. 283–302. Alan R. Liss, New York.Google Scholar
- Galbraith, D.W. 1990. Flow cytometric analysis of plant genomes. In:Methods in Cell Biology (eds. Z. Datzynkiewicz and H. Crissman), pp. 549–563. Academic Press, New York.Google Scholar
- Laurie, D.A. and M.D. Bennett. 1985. Nuclear DNA content in the generaZea andSorghum. Intergeneric, interspecific and intraspecific variation. Heredity 55:307–313.Google Scholar