Plant Molecular Biology Reporter

, Volume 9, Issue 3, pp 208–218 | Cite as

Nuclear DNA content of some important plant species

  • K. Arumuganathan
  • E. D. Earle
Genetic Resources


Nuclear DNA contents of more than 100 important plant species were measured by flow cytometry of isolated nuclei stained with propidium iodide.Arabidopsis exhibits developmentally regulated multiploidy and has a 2C nuclear DNA content of 0.30 pg (145 Mbp/1C), twice the value usually cited. The 2C value for rice is only about three times that ofArabidopsis. Tomato has a 2C value of about 2.0 pg, larger than commonly cited. This survey identified several horticultural crops in a variety of families with genomes only two or three times as large asArabidopsis; these include several fruit trees (a pricot, cherry, mango, orange, papaya, and peach). The small genome sizes of rice and the horticultural plants should facilitate molecular studies of these crops.

Key words

Genome size flow cytometry propidium iodide Arabidopsis rice tomato 



DNA content of the unreplicated haploid chromosome complement


chicken red blood cells


chemical extraction


flow cytometry


Feulgen microdensitometry


reassociation kinetics


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  1. Arumuganathan, K. and E.D. Earle 1991. Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol. Biol. Reporter 9:221–231.Google Scholar
  2. Bennett, M.D. 1985. Intraspecific variation in DNA amount and the nucleotypic dimension in plant genetics. In:Plant Genetics (ed. M. Freeling), pp. 283–302. Alan R. Liss, New York.Google Scholar
  3. Bennett, M.D. and J.B. Smith. 1976. Nuclear DNA amounts in angiosperms. Phil. Trans. R. Soc. Lond. B 274:227–274.CrossRefGoogle Scholar
  4. Bennett, M.D., J.B. Smith and J.S. Heslop-Harrison. 1982. Nuclear DNA amounts in angiosperms. Proc. R. Soc. Lond. B 216:179–199.CrossRefGoogle Scholar
  5. Cameron, M.J. 1990. Macintosh graphics for the EPICS flow cytometer user. Cytometry 11:916–918.PubMedCrossRefGoogle Scholar
  6. De Rocher, E.J., K.R. Harkins, D.W. Galbraith and H.J. Bohnert. 1990. Developmentally regulated systemic endopolyploidy in succulents with small genomes. Science 250:99–101.CrossRefPubMedGoogle Scholar
  7. Galbraith, D.W., K.R. Harkins, J.M. Maddox, N.M. Ayres, D.P. Sharma and E. Firoozabady. 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049–1051.CrossRefPubMedGoogle Scholar
  8. Galbraith, D.W. 1990. Flow cytometric analysis of plant genomes. In:Methods in Cell Biology (eds. Z. Datzynkiewicz and H. Crissman), pp. 549–563. Academic Press, New York.Google Scholar
  9. Iyengar, G.A.S. and S.K. Sen. 1978. Nuclear DNA content of several wild and cultivatedOryza species. Environ. Exp. Bot. 18:219–224.CrossRefGoogle Scholar
  10. Leutwiler, L.S., B.R. Hough-Evans and E.M. Meyerowitz. 1984. The DNA ofArabidopsis thaliana. Mol. Gen. Genet. 194:15–23.CrossRefGoogle Scholar
  11. Laurie, D.A. and M.D. Bennett. 1985. Nuclear DNA content in the generaZea andSorghum. Intergeneric, interspecific and intraspecific variation. Heredity 55:307–313.Google Scholar
  12. Messeguer, R., M.W. Ganal, J.C. Steffens and S.D. Tanksley. 1991. Characterization of the level, target sites, and inheritance of cytosine methylation in tomato nuclear DNA. Plant Mol. Biol. 16:753–770.PubMedCrossRefGoogle Scholar
  13. Michaelson, M.J., H.J. Price, J.R. Ellison and J.S. Johnston. 1991. Comparison of plant DNA contents determined by Feulgen microspectrophotometry and laser flow cytometry. Amer. J. Bot. 78:183–188.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • K. Arumuganathan
    • 1
  • E. D. Earle
    • 1
  1. 1.Department of Plant Breeding and BiometryCornell UniversityIthacaUSA

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