Plant Molecular Biology Reporter

, Volume 9, Issue 3, pp 208–218

Nuclear DNA content of some important plant species

  • K. Arumuganathan
  • E. D. Earle
Genetic Resources

Abstract

Nuclear DNA contents of more than 100 important plant species were measured by flow cytometry of isolated nuclei stained with propidium iodide.Arabidopsis exhibits developmentally regulated multiploidy and has a 2C nuclear DNA content of 0.30 pg (145 Mbp/1C), twice the value usually cited. The 2C value for rice is only about three times that ofArabidopsis. Tomato has a 2C value of about 2.0 pg, larger than commonly cited. This survey identified several horticultural crops in a variety of families with genomes only two or three times as large asArabidopsis; these include several fruit trees (a pricot, cherry, mango, orange, papaya, and peach). The small genome sizes of rice and the horticultural plants should facilitate molecular studies of these crops.

Key words

Genome size flow cytometry propidium iodide Arabidopsis rice tomato 

Abbreviations

C

DNA content of the unreplicated haploid chromosome complement

CRBC

chicken red blood cells

CE

chemical extraction

FC

flow cytometry

FMD

Feulgen microdensitometry

RK

reassociation kinetics

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • K. Arumuganathan
    • 1
  • E. D. Earle
    • 1
  1. 1.Department of Plant Breeding and BiometryCornell UniversityIthacaUSA

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