A protocol for non-radioactive DNA labelling and detection in the RFLP analysis of rice and tomato using single-copy probes
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An improved protocol for non-radioactive labelling and detection of rice and tomato DNA is described. The procedure includes the use of polymerase chain reaction for the incorporation of digoxigenin-dUTP in the DNA molecule and the use of a chemiluminescent compound (AMPPD) for the signal detection.
Key WordsRFLP rice tomato digoxigenin non-radioactive DNA labelling PCR
polymerase chain reaction
restriction fragment length polymorphism
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