Plant Molecular Biology Reporter

, Volume 11, Issue 2, pp 122–127 | Cite as

A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis

  • Andrew H. Paterson
  • Curt L. Brubaker
  • Jonathan F. Wendel


Extraction of high-quality genomic DNA fromGossypium (cotton) species is difficult due to high levels of polysaccharide, oxidizable quinones, and other interfering substances. We describe a procedure that consistently permits isolation of cotton genomic DNA of satisfactory size and quality for RFLP and PCR analysis, as well as for most routine cloning applications. Several antioxidants, phenol-binding reagents, and phenol oxidase inhibitors are used throughout the procedure, and most polysaccharides are eliminated early in the procedure by isolation of nuclei.

Key Words

cotton genomic DNA extraction Gossypium PCR RFLP 



chloroform-isoamyl alcohol


hexadecyltrimethyl-ammonium bromide


diethyldithiocarbamic acid


random amplified polymorphic DNA


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Copyright information

© International Society for Plant Molecular Biology 1993

Authors and Affiliations

  • Andrew H. Paterson
    • 1
  • Curt L. Brubaker
    • 2
  • Jonathan F. Wendel
    • 1
  1. 1.Department of Soil and Crop SciencesTexas A & M UniversityCollege StationUSA
  2. 2.Department of BotanyIowa State UniversityAmesUSA

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