RNA isolation from recalcitrant plant tissue
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The isolation of high-quality RNA from various tissues (leaves, pedicels, glandular trichomes) of garden geranium (Pelargonium xhortorum) using various published methods is difficult due to numerous oxidizing compounds. A new RNA extraction method was developed through the combination and modification of two separate procedures (Rochester et al., 1986; Manning 1991). In addition to geranium tissues, this method is successful when used with other recalcitrant tissues such as mature needles of white pine (Pinus strobus) and mature leaves of poinsettia (Euphorbia pulcherrima). RNA quality was judged by spectrophotometric readings, denaturing agarose gels, and successful reverse transcription.
Key WordsRNA isolation geranium glandular trichomes recalcitrant plant tissue
ethylene glycol-bis (β-aminoethyl ether) N, N, N-, N-tetraacetic acid
ethylene glycol monobutylether
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- Baker, S.S., C.L. Rugh, and J.C. Kamalay. 1990. RNA and DNA isolation from recalcitrant plant tissues. Bio Techniques 9: 268–272.Google Scholar
- Levi, A., G.A. Galau, and H.Y. Wetzstein. 1992. A rapid procedure for the isolation of RNA from high-phenolic-containing tissues of pecan. HortScience 27: 1316–1318.Google Scholar
- Lopez-Gomez, R., and M.A. Gomez-Lim. 1992. A method for extraction intact RNA from fruits rich in polysaccharides using ripe mango mesocarp. HortScience 27: 440–442.Google Scholar
- Rochester, D.E., J.A. Winer, and D.M. Shah. 1986. The structure and expression of maize genes encoding the major heat shock protein, hsp70. EMBO 5: 451–458.Google Scholar