Plant Molecular Biology Reporter

, Volume 10, Issue 1, pp 60–65 | Cite as

Isolation of plant DNA: A fast, inexpensive, and reliable method

  • Pierre Guillemaut
  • Laurence Maréchal-Drouard
Protocol

Abstract

We describe here a simple method to isolate DNA of high molecular weight from a wide variety of plant materials, such as trees, herbaceous plants, cell suspension cultures, calli, seeds, dried embryos, ferns and lichens. The crucial step of the extraction is the use of an acidic extraction medium. When necessary, the sample was separated on a fast RPC-5 column providing us with highly purified DNA suitable not only for restriction endonuclease analyses but also for PCR experiments, RLFP analyses, or detection of adducts.

Key Words

DNA isolation woody plant seed cell culture 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Pierre Guillemaut
    • 1
  • Laurence Maréchal-Drouard
    • 2
  1. 1.Laboratoire de Biologie Végétale AppliquéeInstitut Universitaire de Technologie Louis PasteurStrasbourgFrance
  2. 2.Institut de Biologie Moléculaire des Plantes du CNRSStrasbourg CedexFrance

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