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Plant Molecular Biology Reporter

, Volume 8, Issue 1, pp 8–12 | Cite as

Isolation of DNA from plants high in polyphenolics

  • Jennifer A. Couch
  • Paul J. Fritz
Protocol

Abstract

DNA suitable for digestion with restriction endonucleases has been isolated fromTheobroma cacao, a plant high in polyphenolic compounds, and several other plant taxa using the method presented in this paper. The method relies on concentrating nuclei away from most of the cytoplasmic components and inhibiting the formation of oxidized polyphenolic compounds and their interaction with the DNA fraction in the remaining steps.

Keywords

DNA isolation polyphenols Theobroma cacao 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Jennifer A. Couch
    • 1
    • 2
  • Paul J. Fritz
    • 1
    • 2
  1. 1.ACRI Cocoa Molecular Biology Lab, The Intercollege Program of GeneticsPennsylvania State UniversityUniversity ParkUSA
  2. 2.The Department of Food SciencePennsylvania State UniversityUniversity ParkUSA

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