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Plant Molecular Biology Reporter

, Volume 13, Issue 2, pp 147–155 | Cite as

Detection of DNA polymorphisms within the genusCattleya (Orchidaceae)

  • Michael S. Benner
  • Michael D. Braunstein
  • Michael U. Weisberg
Genetic Resources

Abstract

We have adapted methodology necessary for the detection of molecular polymorphisms in the orchid genusCattleya, namely, randomly amplified polymorphic DNA (RAPD). We report a high level of molecular variability among species; each of eight species examined exhibited a unique DNA fingerprint with nine out of ten arbitrary primers used in single-primer RAPD reactions. Among progeny of an intraspecificCattleya cross, 55 percent of major amplification products were found to segregate. Segregation of these markers facilitated the preliminary identification of several linkage intervals. The identification and mapping of DNA polymorphisms by the RAPD technique will facilitate the use of these taxa for the identification of species-specific and genus-specific traits, allow for the measurement of recombination and introgression in hybrid populations, and enable geneticists to address concordance (or lack thereof) in the processes of speciation, morphological evolution, and molecular change in a large and highly advanced plant family.

Key Words

DNA extraction RAPD-PCR Cattleya Laeliinae Orchidaceae 

Abbreviations

PCR

polymerase chain reaction

RAPD

randomly amplified polymorphic DNA

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Michael S. Benner
    • 1
  • Michael D. Braunstein
    • 1
  • Michael U. Weisberg
    • 1
  1. 1.Department of BiologyRider UniversityLawrencevilleUSA

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